Diagnostic & Veterinary
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BCECF Acid
Descrition & Target
BCECF (2',7' -<i>bis</i>-(Carboxyethyl)-5-(and-6)-carboxyfluorescein) is the most widely used fluorescent pH sensor. Because its pK<sub>a</sub> (6.97) is close to physiological pH, BCECF can detect cytosolic pH change with high sensitivity. At low pH, the dye is weakly fluorescent but becomes more fluorescent with increasing pH. The excitation spectrum of the dye undergoes a slight shift during pH change, while the wavelength of the emission maximum remains unchanged. The pH is determined ratiometrically by the relative fluorescent intensities at 535 nm when the dye is excited at 439 nm and505 nm respectively. BCECF, acid is membrane-impermeant and therefore can be loaded into cells via microinjection or scrape loading. Also see the membrane permeant AM ester form of BCECF.
<ul>
<li>&lamba;<sub>Ex</sub>/&lamba; <sub>Em</sub> = 508nm530nm</li>
<li>Yellow orange solid soluble in DMSO</li>
<li>Store at 4°C and protect from light, especially in solution</li>
<li>MW: 520.5</li>
<li>[85138-49-4]</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
BCECF, AM ESTER
Descrition & Target
BCECF, AM ester is membrane-permeant and thus can be loaded into cells via incubation. This product is a mixture of three molecular species, all of which are readily hydrolyzed by endogenous esterases into a single BCECF free acid form once they are in the cells.
<ul>
<li>White solid soluble in DMSO and DMF</li>
<li>Store at -20°C and protect from light, especially in solution</li>
<li>MW: approx. 615</li>
<li>[117464-70-7]</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
BCECF, AM Ester 1 mg/mL in DMSO
Descrition & Target
BCECF, AM ester is membrane-permeant and thus can be loaded into cells via incubation. This product is a mixture of three molecular species, all of which are readily hydrolyzed by endogenous esterases into a single BCECF free acid form once they are in the cells.
<ul>
<li>White solid soluble in DMSO and DMF</li>
<li>Store at -20°C and protect from light, especially in solution</li>
<li>MW: approx. 615</li>
<li>[117464-70-7]</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
BCECF,AM ESTER,100UG,
Descrition & Target
BCECF, AM ester is membrane-permeant and thus can be loaded into cells via incubation. This product is a mixture of three molecular species, all of which are readily hydrolyzed by endogenous esterases into a single BCECF free acid form once they are in the cells.
<ul>
<li>White solid soluble in DMSO and DMF</li>
<li>Store at -20°C and protect from light, especially in solution</li>
<li>MW: approx. 615</li>
<li>[117464-70-7]</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
BCECF,AM ESTER,50UG
Descrition & Target
BCECF, AM ester is membrane-permeant and thus can be loaded into cells via incubation. This product is a mixture of three molecular species, all of which are readily hydrolyzed by endogenous esterases into a single BCECF free acid form once they are in the cells.
<ul>
<li>White solid soluble in DMSO and DMF</li>
<li>Store at -20°C and protect from light, especially in solution</li>
<li>MW: approx. 615</li>
<li>[117464-70-7]</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression