Descrition & Target

Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* *CAS 136632-31-0*

Descrition & Target

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3' end terminal labeling. The terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies. The number '16' is the number of atoms in the linker between biotin and dUTP. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 also offers biotin-11-dUTP and biotin-20-dUTP. The length of the linker affects the incorporation efficiency of the biotin-dUTP probe into DNA using DNA polymerases, and it also affects biotin/avidin or biotin/streptavidin. In general, the shorter the linker, the more efficiently the biotin-dUTP is incorporated into DNA by DNA polymerases. On the other hand, a longer linker should facilitate interaction between biotin and avidin or streptavidin.
<ul>
<li>1 mM solution in pH 7.5 Tris-HCl buffer</li>
<li>Store at -20°C</li>
<li>C₃₂H₄₈N₇O₁₈P₃Sli₄</li>
<li>MW: 971.5</li>
</ul>
Biotin-16-dUTP is also available as a lyophilized solid.

Descrition & Target

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3' end terminal labeling. The terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies. The number '16' is the number of atoms in the linker between biotin and dUTP. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 also offers biotin-11-dUTP and biotin-20-dUTP. The length of the linker affects the incorporation efficiency of the biotin-dUTP probe into DNA using DNA polymerases, and it also affects biotin/avidin or biotin/streptavidin. In general, the shorter the linker, the more efficiently the biotin-dUTP is incorporated into DNA by DNA polymerases. On the other hand, a longer linker should facilitate interaction between biotin and avidin or streptavidin.

<ul>
<li>Lyophilized solid suitable for long term storage; contains lyophilized TE buffer</li>
<li>Store at -20°C</li>
<li>C₃₂H₄₈N₇O₁₈P₃Sli₄</li>
<li>MW: 971.5</li>
</ul>
Biotin-16-dUTP is also available as a 1 mM solution in pH 7.5 Tris-HCl buffer.

Descrition & Target

This product is similar to biotin-11-UTP and biotin-16-UTP except that it has a longer and water soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.
<ul>
<li>10 mM solution in pH 7.5 Tris-HCl buffer</li>
<li>Store at -20°C</li>
<li>C₃₃H₅₁N₆O₂₂P₃SLi₄</li>
<li>MW: 1036.26</li>
</ul>

Descrition & Target

Biotin-20-dUTP *1 mM in Tris Buffer (pH 7.5)*

Descrition & Target

This product is similar to biotin-11-dUTP and biotin-16-dUTP except that it has a longer and water-soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.
<ul>
<li>1 mM solution in pH 7.5 Tris-HCl buffer</li>
<li>Store at -20°C</li>
<li>C₃₃H₅₁N₆O₂₁P₃SLi₄</li>
<li>MW: 1020.54</li>
</ul>
Biotin-20-dUTP is also available as a lyophilized solid.

Descrition & Target

This product is similar to biotin-11-dUTP and biotin-16-dUTP except that it has a longer and water-soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.
<ul>
<li>Lyophilized solid suitable for long term storage; contains lyophilized TE buffer</li>
<li>Store at -20°C</li>
<li>C₃₃H₅₁N₆O₂₁P₃SLi₄</li>
<li>MW: 1020.54</li>
</ul>
Biotin-20-dUTP is also available as a 1 mM solution in TE buffer.

Descrition & Target

Biotin-4-fluorescein (<i>N</i>-(5-aminopentyl)biotinamide, trifluoroacetic acid salt) has been reported to be a superior fluorescent biotin derivative for accurate measurement of avidin and streptavidin in crude biological samples. Binding of biotin to avidin and streptavidin is high affinity, fast and noncooperative.
<ul>
<li>&lamba;_Ex/&lamba; _Em = 494/523 nm</li>
<li>Yellow solid soluble in DMSO, or pH&gt;7 buffers</li>
<li>Store at 4°C and protect from light</li>
<li>C₃₃H₃₂N₄O₈S</li>
<li>MW: 644.7</li>
</ul>

Descrition & Target

Biotin-4-fluorescein *CAS 1032732-74-3*

Descrition & Target

Biotin-AM3-25 is a conjugate of AM3-25 and biotin. Membranes labeled with the probe can subsequently couple to avidin or streptavidin.
<ul>
<li>&lamba;_Ex/&lamba; _Em (MeOH) = 510/625 nm; &lamba; _Ex/&lamba; _Em (liposomes) = 480/600 nm</li>
<li>Dark red soluble in MeOH or DMSO</li>
<li>Store at -20°C and protect from light</li>
<li>C₆₇H₁₁₈Cl₂N₆O₂S</li>
<li>MW: 1142.66</li>
</ul>

Descrition & Target

Potential applications of Biotin-CF&reg; dye include detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples. In addition, biotin-CF dye can be used as a polar tracer by microinjection to study the morphology of cells. CF&reg; dyes are very photostable which make them suitable for studies where prolonged exposure to light may be necessary.

Descrition & Target

Potential applications of Biotin-CF&reg; dye include detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples. In addition, biotin-CF&reg; dye can be used as a polar tracer by microinjection to study the morphology of cells. CF&reg; dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF&reg;568 dye has excitation/emission at 562/583 nm.

Descrition & Target

Potential applications of Biotin-CF&reg; dye include detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples. In addition, biotin-CF&reg; dye can be used as a polar tracer by microinjection to study the morphology of cells. CF&reg; dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF&reg;640R dye has excitation/emission at 642/662 nm.

Descrition & Target

Biotin-DHPE (N-(Biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.
<ul>
<li>White solid soluble in chloroform</li>
<li>Store at -20°C</li>
<li>C₅₃H₁₀₃N₄O₁₀PS</li>
<li>MW: 1019.44</li>
<li>[136235-58-0]</li>
</ul>

Descrition & Target

Biotin-PEG3-azide *CAS 945633-30-7*