Diagnostic & Veterinary
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1X Passive Lysis Buffer 2.0
Descrition & Target
1X Passive Lysis Buffer 2.0 for 9689c5aa-6556-4b3c-b5d1-1e693715ba07’s Firefly & Renilla Luciferase Single Tube Assay Kits (catalog no. 30081) is sold separately for use with the kits when more lysis buffer (e.g. >100 uL/well) is required. It also can be used with recombinant firefly or Renilla luciferase enzymes.
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2',7'-DICHLORODIHYDROFluorescein DIACETA
Descrition & Target
H<sub>2</sub>DCFDA (2',7'-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H<sub>2</sub>DCFDA becomes the highly green fluorescent 2',7'-dichlorofluorescein.<ul><li>λ<sub>Ex</sub>/λ <sub>Em </sub>(pH>6) = 504/529 nm (end product)</li><li>White solid soluble in DMSO or DMF</li><li>Store desiccated at -20°C and protect from light</li><li>C<sub>24</sub>H<sub>16</sub>Cl<sub>2</sub>O<sub>7</sub></li><li>MW: 487.29</li><li>[4091-99-0]</li></ul>
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2,3-Diaminonaphthalene
Descrition & Target
2,3-Diaminonaphthalene reacts with nitrosonium, which is formed from NO, to form the fluorescent dye 1 <i>H</i>-naphthotriazole. This method can be used to detect 10 nM to 10 uM of nitrite (NO<sub>2</sub>-) and is compatible with 96-well format. <ul> <li>Yellow orange solid soluble in DMSO</li> <li>Store at desiccated at 4°C, protect from light</li> <li>C<sub>10</sub>H<sub>10</sub>N<sub>2</sub></li> <li>MW: 158</li> </ul>
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