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CF®647 alkyne
Descrition & Target
CF® dye alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form fluorescent polymers. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®640R dye has excitation/emission at 642/662 nm.
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CF®647 amine
Descrition & Target
CF® Dye Amines can be used to conjugate CF® dyes to carboxylic acids groups in biological molecules. Carboxylic acids can be activated using water soluble carbodiimides to react with amine-functionalized dyes to form a stable amide bond. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®647 dye has excitation/emission at 650/665 nm.
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CF®647 azide
Descrition & Target
CF® dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®647 dye has excitation/emission at 650/665 nm.
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CF®647 dCTP, Lyophilized Powder
Descrition & Target
CF® dye dCTP can be used to synthesize labeled DNA probes for in situ hybridization, microarray or blotting applications. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®647 dye has excitation/emission at 650/665 nm.
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CF®647 hydrazide
Descrition & Target
Our CF® dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for microinjection into cells. Hydrazides also can be used for labeling aldehyde or ketone groups, however, for this application we recommend using the more reactive CF® dye aminooxy forms. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®647 dye has excitation/emission at 650/665 nm.
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CF®647 maleimide
Descrition & Target
CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins on free thiol groups. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®647 dye has excitation/emission at 650/665 nm.
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CF®647 methyltetrazine
Descrition & Target
CF® dye with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) containing molecules via copper free click chemistry. This copper free bioorthogonal reaction is useful for labeling live cells, or when there are concerns about native protein function loss with copper in cell extracts. Methyltetrazine has improved chemical stability compared to tetrazine. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®647 dye has excitation/emission at 650/665 nm.
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CF®647 picolyl azide
Descrition & Target
CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®647 dye has excitation/emission at 650/665 nm.
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CF®647 tetrazine
Descrition & Target
CF® dye with tetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry. This copper free bioorthogonal reaction is useful for staining the surface of live cells or when there are concerns about native protein function loss with copper in cell extracts. Tetrazine allows faster reaction kinetics than methyltetrazine. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®647 dye has excitation/emission at 650/665 nm.
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CF®647 tyramide
Descrition & Target
CF® dye tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®647 dye has excitation/emission at 650/665 nm.
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CF®647, succinimidyl ester
Descrition & Target
CF® Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®647 dye has excitation/emission at 650/665 nm.
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CF®647-dCTP, Lyophilized Powder
Descrition & Target
CF® dye dCTP can be used to synthesize labeled DNA probes for in situ hybridization, microarray or blotting applications. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®647 dye has excitation/emission at 650/665 nm.
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CF®647DI Aminooxy
Descrition & Target
Aminooxy dyes have an aminooxy reactive group, which can be used to label a target molecule with an aldehyde or ketone group. An aminooxy group readily reacts with an aldehyde or ketone group to form a stable linkage without the use of a reducing agent.
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CF®647DI hydrazide
Descrition & Target
CF®DI dye hydrazide could be used to react with aldehyde or ketone groups from protein carbonylation. CF®DI dyes are designed to match for charge and molecular weight for 2D DIGE platform. Far-red fluorescent CF®647DI has excitation/emission at 639/668 nm.
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CF®650 BCN
Descrition & Target
CF® dye with BCN reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper-free bioorthogonal reaction allows staining the surface of live cells or cell extracts when there are concerns about native protein function loss with copper. CF®650-BCN has excitation/emission at 650/670 nm, and is designed for intracellular copper-free reaction with azide.
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