Diagnostic & Veterinary
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Calcein (High Purity)
Descrition & Target
Calcein is a highly negatively charged, water-soluble fluorescein derivative. Its fluorescence is nearly independent of pH in the range between 6.5 and 12. The dye can be introduced into cells by microinjection and is useful for cell tracing and for the study of cell membrane integrity and fusion. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 also offers calcein AM, the membrane-permeant version of calcein that hydrolyzes into calcein once inside cells.
<ul>
<li>&lamba;Ex/&lamba;Em = 494/517 nm (pH 8).</li>
<li>ε = 75,000</li>
<li>Yellow-orange solid soluble in DMSO, DMF or pH>6 water</li>
<li>Store at 4°C and protect from light</li>
<li>C<sub>30</sub>H<sub>26</sub>N<sub>2</sub>O<sub>13</sub></li>
<li>MW: 623</li>
<li>[154071-48-4]</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
Calcein AM
Descrition & Target
Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation. Once inside the cells, calcein AM is hydrolyzed by endogenous esterase into the highly negatively charged green fluorescent calcein, which is retained in the cytoplasm. Calcein AM is an excellent tool for the studies of cell membrane integrity and for cell tracing. Supplied in lyophilized form or as a solution in anhydrous DMSO at 4 mM (80011-1) or 1 mg/mL (1 mM) (80011-2). Calcein AM is also offered in our Cell Viability and Cytotoxicity Assay Kit.
<ul>
<li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em</sub> (calcein) = 494/517 nm (pH 8)</li>
<li>Colorless solid soluble in DMSO</li>
<li>Store at -20°C and protect from light</li>
<li>C<sub>46</sub>H<sub>46</sub>N<sub>2</sub>O<sub>23</sub></li>
<li>MW: 995</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
Calcein AM Cell Viability Assay Kit (1000 assays)
Descrition & Target
Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation. Once inside the cells, calcein AM, a nonfluorescent molecule itself, is hydrolyzed by endogenous esterase into the highly negatively charged green fluorescent calcein. Green fluorescent calcein is retained in the cytoplasm in live cells. Calcein AM has served as a useful tool for the studies of cell membrane integrity and for cell tracing. The Calcein AM Cell Viability Assay Kit is designed to quantify live cell numbers based on their endogenous esterase activity and plasma membrane integrity. It is a true end-point assay for cell viability. The fluorescent signal is monitored using 485 nm excitation wavelength and 530 nm emission wavelength. The fluorescent signal generated from the assay is proportional to the number of living cells in the sample.
<p class="style5 style11"><strong>Kit Components: </strong></p>
<ul>
<li>Calcein AM in anhydrous DMSO</li>
</ul>
<strong>Features:</strong>
<ul>
<li><b>True end-point viability assay:</b> Detect only live cells with intact plasma membrane integrity</li>
<li><b>Simple & Fast</b>: 30-min assay</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
Calcein AM, 1 mg/mL in Anhydrous DMSO
Descrition & Target
Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation. Once inside the cells, calcein AM is hydrolyzed by endogenous esterase into the highly negatively charged green fluorescent calcein, which is retained in the cytoplasm. Calcein AM is an excellent tool for the studies of cell membrane integrity and for cell tracing. Supplied in lyophilized form or as a solution in anhydrous DMSO at 4 mM (80011-1) or 1 mg/mL (1 mM) (80011-2). Calcein AM is also offered in our Cell Viability and Cytotoxicity Assay Kit.
<ul>
<li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em</sub> (calcein) = 494/517 nm (pH 8)</li>
<li>Colorless solid soluble in DMSO</li>
<li>Store at -20°C and protect from light</li>
<li>C<sub>46</sub>H<sub>46</sub>N<sub>2</sub>O<sub>23</sub></li>
<li>MW: 995</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
Calcein AM, 4 mM in Anhydrous DMSO
Descrition & Target
Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation. Once inside the cells, calcein AM is hydrolyzed by endogenous esterase into the highly negatively charged green fluorescent calcein, which is retained in the cytoplasm. Calcein AM is an excellent tool for the studies of cell membrane integrity and for cell tracing. Supplied in lyophilized form or as a solution in anhydrous DMSO at 4 mM (80011-1) or 1 mg/mL (1 mM) (80011-2). Calcein AM is also offered in our Cell Viability and Cytotoxicity Assay Kit.
<ul>
<li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em</sub> (calcein) = 494/517 nm (pH 8)</li>
<li>Colorless solid soluble in DMSO</li>
<li>Store at -20°C and protect from light</li>
<li>C<sub>46</sub>H<sub>46</sub>N<sub>2</sub>O<sub>23</sub></li>
<li>MW: 995</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
Calcein AM, Special Packaging, 20x50 ug
Descrition & Target
Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation. Once inside the cells, calcein AM is hydrolyzed by endogenous esterase into the highly negatively charged green fluorescent calcein, which is retained in the cytoplasm. Calcein AM is an excellent tool for the studies of cell membrane integrity and for cell tracing. Supplied in lyophilized form or as a solution in anhydrous DMSO at 4 mM (80011-1) or 1 mg/mL (1 mM) (80011-2). Calcein AM is also offered in our Cell Viability and Cytotoxicity Assay Kit.
<ul>
<li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em</sub> (calcein) = 494/517 nm (pH 8)</li>
<li>Colorless solid soluble in DMSO</li>
<li>Store at -20°C and protect from light</li>
<li>C<sub>46</sub>H<sub>46</sub>N<sub>2</sub>O<sub>23</sub></li>
<li>MW: 995</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression