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DNP-X acid, SE [6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester] *CA
Descrition & Target
DNP-X acid, SE [6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester] *CAS 82321-04-8*
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DODC (DiOC2(5)) (high purity)
Descrition & Target
DODC (DiOC<sub>2</sub>(5)) has been reported to bind to triplex DNA as well as dimeric hairpin quadruplex DNA. Binding of the dye to triplex DNA quenches the fluorescence of the dye and red-shifts the dye absorption peak. <ul> <li>&lamba;A<sub>bs</sub> = 582 nm</li> <li>Red solid soluble in DMSO or MeOH</li> <li>Store at 4°C</li> <li>C<sub>23</sub>H<sub>23</sub>IN<sub>2</sub>O<sub>2</sub></li> <li>MW: 486.35</li> </ul>
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DPA
Descrition & Target
Dipicrylamine (DPA) is a nonfluorescent anionic membrane dye. With its absorbance in the blue region of the visible spectrum, DPA can be used as a FRET quencher for a variety of fluorescent donor dyes. When applied to cells, DPA localizes at the interface of cytoplasmic membrane and aqueous phase in a manner sensitive to membrane potential change. A recent application of DPA is to measure cell membrane potential change by combining with the fluorescent donor dye DiOC16. The DiO/DPA system has been reported to produce a fluorescence signal change of >56% in HEK-293 cells and >25% in neuronal cultures and brain slices per 100 mV membrane potential change. Please also see the DiO/DPA Membrane Potential Detection Kit.
Yellow solid soluble in DMSO at >30mg/mL
<ul>
<li>&lamba;abs (MeOH) = 406 nm</li>
<li>Store at 4°C</li>
<li>MWt: 439.21</li>
<li>[131-73-7]</li>
</ul>
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DPA/Terbium for membrane fusion assay
Descrition & Target
The principle of DPA/Tb<sup>3 </sup> for vesicle fusion assay is based on the fact that contact of the chelator dipicolinic acid (DPA) with terbium (III) forms an instant Tb<sup>3 </sup>-DPA complex that is <em>~</em>10,000 times more fluorescent than free Tb<sup>3 </sup>. In the assay, separate vesicle populations are loaded with 2.5 mM TbCl3 in 50 mM sodium citrate, or 50 mM DPA in 20 mM NaCl. Fusion of the two types of vesicles results in fluorescence increase at 490 nm or 545 nm, with excitation at 276 nm. Each set of product contains 1 g terbium trichloride and 1 g DPA in two separate vials. Please also see SDIP/Europium for membrane fusion assay, which results in intense red fluorescence upon complex formation.
<ul>
<li>&lamba;Ex/&lamba;Em (DPA/Tb<sup>3</sup> complex) = 276/490 and 545 nm</li>
<li>DPA and Terbium are white solids and readily soluble in water</li>
<li>Store both reagents at room temperature</li>
<li>MW of DPA: 167.12</li>
<li>CAS#: 499-83-2</li>
<li>MW of Terbium: 265.3</li>
<li>CAS#:13798-24-8</li>
</ul>
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DPX
Descrition & Target
DPX is a positively charged quencher that is often used with ANTS to study membrane fusion or permeability.
<ul>
<li>Off-white solid soluble in water</li>
<li>Store desiccated at 4°C</li>
<li>C<sub>18</sub>H<sub>18</sub>Br<sub>2</sub>N<sub>2</sub></li>
<li>MW: 422</li>
<li>[14208-10-7]</li>
</ul>
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DRAM2 (DNA Damage-regulated Autophagy Modulator Protein 2, Transmembrane Protein
Descrition & Target
Collagen determination in biological and cosmetic products by fluorimetric (375/465nm) method. Procedure: 80 min. Kit size: 100 tests. Detection limit: 2 µg/mL to 50 µg/mL collagen. Shelf life: 6 months. Shipping: on ice; storage: -20°C.
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DTT
Descrition & Target
DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.
<ul>
<li>White solid soluble in water (5% w/v)</li>
<li>Store at -20°C</li>
<li>C<sub>4</sub>H<sub>10</sub>O<sub>2</sub>S<sub>2</sub></li>
<li>MW: 154.2</li>
<li>[3483-12-3]</li>
</ul>
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DUSP21 ((Dual Specificity Protein Phosphatase 21, Low Molecular Weight Dual Spec
Descrition & Target
Quantitative determination of cholesterol by colorimetric (570nm) or fluorometric (530nm/590nm) methods. Procedure: 40 min. Kit size: 100 tests. Detection limit: colorimetric assay 1 mg/dL, fluorimetric assay 0.2 mg/dL. Shelf life: 6 months. Shipping: on ice; storage: -20°C.
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