Diagnostic & Veterinary
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DiIC12(5)-DS [1,1-Diododecyl-3,3,3,3-tetramethylindodicarbocyanine-5,5-disulfoni
Descrition & Target
DiIC12(5)-DS [1,1-Diododecyl-3,3,3,3-tetramethylindodicarbocyanine-5,5-disulfonic acid]
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DiIC16(3) perchlorate [1,1-Dihexadecyl-3,3,3,3-tetramethylindocarbocyanine perch
Descrition & Target
DiIC16(3) perchlorate [1,1-Dihexadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate]
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DiIC18(3)-DS [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-5,5-disulfonic
Descrition & Target
DiIC18(3)-DS [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-5,5-disulfonic acid]
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DiIC18(5)-DS [1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine-5,5-disulfon
Descrition & Target
DiIC18(5)-DS [1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine-5,5-disulfonic acid]
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DiO/DPA membrane potential detection kit
Descrition & Target
The DiO/DPA system detects cytoplasmic membrane potential changes using the principle of fluorescence resonance energy transfer (FRET), where DiO is a "stationary" FRET donor while DPA acts as a potentiometric FRET acceptor. The DiO/DPA system has been reported to produce a fluorescence signal change of >56% in HEK-293 cells and >25% in neuronal cultures and brain slices per 100 mV membrane potential change. The DiO/DPA membrane potential detection kit includes a vial of the green fluorescent membrane dye DiOC16(3) in DMSO (1 mL at 2 mM) and a vial of the anionic membrane quencher dye DPA (dipicrylamine) in DMSO (1 mL at 20 mM).
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DiOC16(3)
Descrition & Target
DiOC16(3) is a lipophilic cyanine dye with two C16 hydrocarbon chains. It can be optimally excited by the 488 nm argon laser line with green fluorescence emission (&lamda;Ex/&lamba;Em = 484/501 nm). The dye can be used to stain cytoplasmic membranes. Recently, DiOC16 has been combined with DPA to detect cell membrane potential change with high sensitivity. Please also see the DiO/DPA Membrane Potential Detection Kit (30037)).
<ul>
<li>Orange solid soluble in DMF and DMSO at >5mg/mL</li>
<li>&lamba;Abs/&lamba;Em (MeOH) = 484/501 nm</li>
<li>ε = ~130,000</li>
<li>Store at 4°C and protect from light</li>
<li>C<sub>55</sub>H<sub>88</sub>N<sub>2</sub>O<sub>9</sub></li>
<li>MWt: 921.30</li>
<li>[ 161433-32-1]</li>
</ul>
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DiOC2(3) (3,3’-Diethyloxacarbocyanine, iodide)
Descrition & Target
DiOC<sub>2</sub>(3) (3,3'-Diethyloxacarbocyanine, iodide) has been used for measuring membrane potentials in bacteria. The green fluorescent dye forms red fluorescent aggregates with increasing membrane potential. The spectral shifting phenomenon has been exploited to measure membrane potential ratiometrically.
<ul>
<li>&lamda;Ex/&lamba;Em (MeOH) = 482/497 nm</li>
<li>Orange solid soluble in DMSO or DMF</li>
<li>Store at 4°C and protect from light</li>
<li>C<sub>21</sub>H<sub>21</sub>IN<sub>2</sub>O<sub>2</sub></li>
<li>MW: 460.31</li>
<li>[905-96-4]</li>
</ul>
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DiOC5(3) (3,3’-Dipentyloxacarbocyanine, iodide)
Descrition & Target
DiOC5(3) and DiOC6(3) are two of the most widely used carbocyanine dyes used for membrane potential measurements.
<ul>
<li>&lamba;Ex/&lamba;Em(MeOH) = 482/497 nm</li>
<li>Orange solid soluble in DMSO or DMF</li>
<li>Store at 4°C and protect from light</li>
<li>C<sub>27</sub>H<sub>33</sub>IN<sub>2</sub>O<sub>2</sub></li>
<li>MW: 544.47</li>
<li>[53213-81-3 ]</li>
</ul>
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