Diagnostic & Veterinary
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5-CR110-PEO3-amine, TFA salt
Descrition & Target
5-CR110-PEO<sub>3</sub>-amine has a long water-soluble PEG spacer that separates the green fluorescent CR110 and the amine functional group. <ul> <li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em </sub>(MeOH) = 502/524 nm</li> <li>Orange yellow solid soluble in DMSO, DMF, or H2O</li> <li>Store at -20°C and protect from light</li> <li>C<sub>33</sub>H<sub>34</sub>F<sub>6</sub>N<sub>4</sub>O<sub>11</sub></li> <li>MW: 776.63</li> </ul>
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5-CR110-PEO4-PROPIONATE SUCCINIMIDYL EST
Descrition & Target
5-CR110-PEO<sub>4</sub> SE (full name: 5-Carboxyrhodamine 110-PEO<sub>4</sub>-propionate, succinimidyl ester, single isomer) is an excellent green fluorescent rhodamine dye for labeling amines. The dye has better solubility than the parent dye 5-CR110 SE and its long PEG spacer should reduce any steric effect of the dye on the molecule to be labeled. Carboxyrhodamine 110 and its derivatives are, in general, significantly more photostable than any other known green fluorescent dyes including Alexa Fluor® 488. In addition, their spectra are unaffected by pH change from 4 to 9. Unlike Alexa Fluor® 488, which is readily degraded under alkaline conditions, CR110 and its derivatives are highly stable under both acidic and basic conditions. As a result, CR110 and its derivatives are superior alternatives to other green fluorescent dyes for applications where only a single dye is required for the labeling. <ul> <li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em </sub>(MeOH) = 502/524 nm</li> <li>Orange red solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from light</li> <li>C<sub>36</sub>H<sub>38</sub>N<sub>4</sub>O<sub>12</sub></li> <li>MW: 718.71</li> </ul>
Alexa Fluor is a registered trademark of Thermo Fisher Scientific.
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5-CR110-X, SE
Descrition & Target
5-CR110-X, SE (full name: 5-Carboxyrhodamine 110-X, succinimidyl ester) is the amine-reactive form of 5-carboxyrhodamine 110 with a 6-carbon spacer. <ul> <li>&lamba;<sub>Ex</sub>/&lamba; <sub>Em </sub>(MeOH) = 502/524 nm</li> <li>Orange red solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C<sub>31</sub>H<sub>28</sub>N<sub>4</sub>O<sub>8</sub></li> <li>MW: 584.58</li> </ul>
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5-Carboxy-2',7'-Dichlorofluorescein (Sin
Descrition & Target
This dye can be used as a low pH indicator similar 5-and-6-Carboxy-2'7'-dichlorofluorescein, and is also useful as a general green fluorescent label. Carboxy-2',7'-dichlorofluorescein is significantly more photostable than carboxyfluorescein.
<ul>
<li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em</sub> = 504/529 nm (pH 8)</li>
<li>ε = 90,000</li>
<li>Yellow solid soluble in DMSO, DMF or basic water (pH >7)</li>
<li>Store at 4°C and protect from light</li>
<li>C<sub>21</sub>H<sub>10</sub>Cl<sub>2</sub>O<sub>7</sub></li>
<li>MW: 445</li>
</ul>
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5-Carboxy-2',7'-dichlorosulfonefluorescein (single isomer)
Descrition & Target
The pKa of 5-carboxy-2',7'-dichlorosulfonefluorescein is near 4 and thus the dye is potentially an excellent pH indicator for acidic organelles.
<ul>
<li>&lamba;<sub>Ex</sub>/&lamba; <sub>Em</sub>= 509/532nm (pH 9)</li>
<li>ε = 92,000</li>
<li>Yellow solid soluble in DMSO, DMF or a buffer (pH >6)</li>
<li>Store at 4°C and protect from light</li>
<li>C<sub>20</sub>H<sub>10</sub>Cl<sub>2</sub>O<sub>8</sub>S</li>
<li>MW: 481.26</li>
</ul>
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5-Carboxy-X-Rhodamine-PEO8-propionate, succinimidyl ester (5-ROX-PEO8 SE)
Descrition & Target
5-ROX-PEO<sub>8</sub> SE (full name: 5-Carboxy-X-rhodamine-PEO<sub>8</sub>-propionate, succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates the red fluorescent ROX dye and the amine-reactive SE group. <ul> <li>&lamba;<sub>Ex</sub>/&lamba; <sub>Em</sub>(MeOH) = 568/595 nm</li> <li>Dark red solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C<sub>56</sub>H<sub>70</sub>N<sub>4</sub>O<sub>16</sub></li> <li>MW: 1055.17</li> </ul>
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5-FAM (5-Carboxyfluorescein)
Descrition & Target
5-FAM (full name: 5-Carboxyfluorescein, single isomer) is the free acid form of 5-carboxyfluorescein single isomer.
<ul>
<li>&lamba;Ex/λEm (pH 9) = 492/514 nm</li>
<li>Yellow orange solid soluble in pH>6 water</li>
<li>Store at 4°C and protect from light, especially in solution</li>
<li>C<sub>21</sub>H<sub>12</sub>O<sub>7</sub></li>
<li>MW: 376.3</li>
<li>[76823-03-5]</li>
</ul>
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