Diagnostic & Veterinary
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6X DNA Loading Buffer (Blue)
Descrition & Target
6X DNA Loading Buffer with Blue Tracking Dyes contains density agents and two blue tracking dyes that run around 1.5 kb and 200 bp in a 1% agarose gel. Mix 1 uL of 6X Loading Buffer for every 5 uL DNA sample before gel loading. 6X DNA Loading Buffer does not contain fluorescent DNA dye. 4 x 1.5 mL.
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6X GelRed® Prestain Buffer with Blue Tracking Dyes
Descrition & Target
GelRed® is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed® Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed® dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting.
The 6X GelRed® Prestain Loading Buffers are available with blue tracking dyes or with orange tracking dye. Prestain buffer with blue tracking dyes (cat. no. 41009) contains two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel. Prestain buffer with orange tracking dye (cat. no. 41010) contains an orange electrophoresis tracking dye that runs at approximately 50 bp in a 1% agarose gel.
GelRed® and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed® without changing your existing imaging system. In addition, GelRed® is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed® is compatible with downstream applications such as sequencing and cloning. GelRed® is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.
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6X GelRed® Prestain Buffer with Orange Tracking Dye
Descrition & Target
GelRed® is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed® Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed® dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting. Download the product protocol on this page for a comparison of prestain, precast, and post-stain protocols for GelRed®, and to find the recommended method for your application.
The 6X GelRed® Prestain Loading Buffers are available with blue tracking dyes or with orange tracking dye. Prestain buffer with blue tracking dyes (cat. no. 41009) contains two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel. Prestain buffer with orange tracking dye (cat. no. 41010) contains an orange electrophoresis tracking dye that runs at approximately 50 bp in a 1% agarose gel.
GelRed® and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed® without changing your existing imaging system. In addition, GelRed® is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed® is compatible with downstream applications such as sequencing and cloning. GelRed® is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.
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7-AAD (7-aminoactinomycin D)
Descrition & Target
7-AAD is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from viable cells. The dye is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity.
7-AAD/DNA complexes can be excited at 488 nm with an argon-ion laser, and has a large Stokes shift with an emission maxima of 647 nm. 7-AAD intercalates selectively at GC-rich regions of DNA, which makes the dye useful for chromosome banding studies. 7-AAD can be used in multicolor fluorescence microscopy and flow cytometry.
<ul>
<li>&lamba;<sub>Ex</sub>/&lamba; <sub>Em</sub>(DNA) = 546/647 nm (7-AAD can also be excited by the 488 or 532 laser lines)</li>
<li>Orange red solid soluble in DMF or DMSO</li>
<li>Store at -20°C and protect from light, especially in solution</li>
<li>C<sub>62</sub>H<sub>87</sub>N<sub>13</sub>O<sub>16</sub></li>
<li>MW: 1270.45</li>
</ul>
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