Diagnostic & Veterinary
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AntiFix™ Universal Antigen Retreival Buffer, 10X, 50 mL
Descrition & Target
AntiFix™ Universal Antigen Retrieval Buffer provides excellent unmasking for a wide variety of targets at neutral pH. It has been validated for fluorescence staining of FFPE tissue with our next-generation fluorescent CF® dyes using HRP-mediated tyramide signal amplification. 50 mL of 10X buffer.
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AntiFix™ Universal Antigen Retreival Buffer, 10X, 500 mL
Descrition & Target
AntiFix™ Universal Antigen Retrieval Buffer provides excellent unmasking for a wide variety of targets at neutral pH. It has been validated for fluorescence staining of FFPE tissue with our next-generation fluorescent CF® dyes using HRP-mediated tyramide signal amplification. 500 mL of 10X buffer.
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Molecular Weight
Molecular Formula
Tag / Host
Expression
Apoptosis & Necrosis Quantification Kit (50 assays)
Descrition & Target
Apoptosis and necrosis are two major processes by which cells die. Apoptosis is an active, genetically regulated disassembly of the cell from within. During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer surface of the cell for phagocytic cell recognition. The human anticoagulant Annexin V is a 35 kDa, Ca<sup>2 </sup>-dependent phospholipid-binding protein with a high affinity for PS. Annexin V labeled with fluorescein (FITC) (λ<sub>Ex</sub>/λ<sub>Em</sub> = 492/514 nm) can identify apoptotic cells in green by binding to PS exposed on the outer leaflet. Necrosis normally results from a severe cellular insult, resulting in loss of plasma membrane integrity. Ethidium homodimer III (EthD-III) is a highly positively charged nucleic acid probe, which is impermeant to live cells or apoptotic cells, but stains necrotic cells with red fluorescence (λ<sub>Ex</sub>/λ<sub>Em</sub> (with DNA) = 528/617 nm). EthD-III is a superior alternative to propidium iodide (PI) or ethidium homodimer I because of its significantly higher affinity for DNA and higher fluorescence quantum yield. The Apoptosis & Necrosis Quantification Kit provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.
<p class="style5 style11"><strong>Kit Components: </strong></p>
<ul>
<li>FITC-Annexin V</li>
<li>Ethidium homodimer III</li>
<li>5X binding buffer</li>
</ul>
<p class="style5 style11"><strong>Features:</strong></p>
<ul>
<li><b>Simple:</b> Simultaneous detection of both apoptotic and necrotic cells in a single assay</li>
<li><b>Fast:</b> A single 15-30 min staining step</li>
<li><b>Sensitive:</b> EthD-III is a superior alternative to both PI and EthD-I for staining necrotic cells.</li>
</ul>
Also see the Apoptosis and Necrosis Quantitation Kit Plus, and Apoptotic, Necrotic, and Healthy Cells Quantitation Kit Plus, which feature Annexin V conjugated to 9689c5aa-6556-4b3c-b5d1-1e693715ba07's superior green fluorescent dye CF®488A, which is brighter and more photostable than FITC.
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
Apoptosis and Necrosis Quantitation Kit Plus (50 assays)
Descrition & Target
Apoptosis and necrosis are two major processes by which cells die. Apoptosis is an active, genetically regulated disassembly of the cell from within. During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane for phagocytic cell recognition. The human anticoagulant Annexin V is a 35 kDa, Ca<sup>2 </sup>-dependent phospholipid-binding protein with high affinity for PS. Annexin V labeled with CF®488A (Abs/Em: 490/515 nm) stains apoptotic cells green by binding to PS exposed on the cell surface. CF®488A is spectrally similar to fluorescein (FITC), with much brighter and more photostable fluorescence.
Necrosis normally results from severe cellular insult. Both internal organelle and plasma membrane integrity are lost, resulting in the release of cellular contents into the surrounding environment. Ethidium homodimer III (EthD-III) is a highly positively charged nucleic acid probe, which is impermeant to live cells, but stains cells with compromised membrane integrity with red fluorescence (Abs/Em (with DNA): 528/617 nm). EthD-III is a superior alternative to propidium iodide (PI) or ethidium homodimer I because of its significantly higher affinity for DNA and higher fluorescence quantum yield. The Apoptosis & Necrosis Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.
<p class="style5 style11"><strong>Kit Components: </strong></p> <ul> <li>CF®488A-Annexin V</li> <li>Ethidium homodimer III</li> <li>5X binding buffer</li> </ul> <p class="style5 style11"><strong>Features:</strong></p> <ul> <li><b>Simple:</b> Simultaneous detection of both apoptotic and necrotic cells in a single assay</li> <li><b>Fast:</b> A single 15-30 min staining step</li> <li><b>Sensitive:</b> CF®488A-Annexin V is brighter and more photostable than FITC-Annexin V, while EthD-III is a superior alternative to both PI and EthD-I for staining necrotic cells.</li> </ul> <p class="style5 style11"></p>
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Apoptotic, Necrotic & Healthy Cells Quantification Kit (50 assays)
Descrition & Target
Apoptosis and necrosis are two major processes by which cells die. Apoptosis is an active, genetically regulated disassembly of the cell from within. During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer surface of the cell for phagocytic cell recognition. The human anticoagulant Annexin V is a 35 kDa, Ca<sup>2 </sup>-dependent phospholipid-binding protein with a high affinity for PS. Annexin V labeled with fluorescein (FITC) (λ<sub>Ex</sub>/λ<sub>Em</sub> = 492/514 nm) can identify apoptotic cells in green by binding to PS exposed on the outer leaflet. Necrosis normally results from a severe cellular insult, resulting in loss of plasma membrane integrity. Ethidium homodimer III (EthD-III) is a highly positively charged nucleic acid probe, which is impermeant to live cells or apoptotic cells, but stains necrotic cells with red fluorescence (λ<sub>Ex</sub>/λ<sub>Em</sub> (with DNA) = 528/617 nm). EthD-III is a superior alternative to propidium iodide (PI) or ethidium homodimer I because of its significantly higher affinity for DNA and higher fluorescence quantum yield. The kit also includes the membrane-permeant blue fluorescent DNA dye Hoechst 33342 (λ<sub>Ex</sub>/λ<sub>Em</sub> (with DNA) = 350/461nm) for visualizing all cells. The kit allows simultaneous quantification of apoptotic (green), necrotic (red) and all cells (blue) within the same cell population by flow cytometry or fluorescence microscopy.
<p class="style5 style11"><strong>Kit Components: </strong></p>
<ul>
<li>FITC-Annexin V</li>
<li>Ethidium Homodimer III</li>
<li>Hoechst 33342</li>
<li>5X binding Buffer</li>
</ul>
<p class="style5 style11"><strong>Features:</strong></p>
<ul>
<li><b>Simple: </b>Simultaneous detection of apoptotic, necrotic and healthy cells in a single assay</li>
<li><b>Fast:</b> A single 15-30 min staining step</li>
<li><b>Sensitive:</b> EthD-III is a superior alternative to both PI and EthD-I for staining necrotic cells.</li>
</ul>
Also see the Apoptosis and Necrosis Quantitation Kit Plus, and Apoptotic, Necrotic, and Healthy Cells Quantitation Kit Plus, which feature Annexin V conjugated to 9689c5aa-6556-4b3c-b5d1-1e693715ba07's superior green fluorescent dye CF®488A, which is brighter and more photostable than FITC.
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression