Fluorescent Labeling: Options & Optimization Even aside from primary factors such as color and affinity, selecting an ideal fluorescent dye in the laboratory often includes attention to current imaging equipment, price, and other practical concerns. For exact comparisons with older publications, Alexa Fluor® conjugates and analogs are a common choice. The expanded iFluor™ series are a popular selection for scientists prioritizing photostability and brightness. For affordability, classic dyes like fluoresceins, cyanines, and others are useful on a large scale, for a moderate loss of performance. Laboratories with time constraints might be best served by antibody and protein labeling kits, designed for easy use with minimal training required. Choose the best option to fit your needs, or contact us for assistance with dye selection. Click To Explore The iFluor™ Catalog
HeLa cells stained with mouse anti-tubulin followed with iFluor™ 594 goat anti-mouse IgG. Actin filaments were stained with Phalloidin-iFluor™ 488 Conjugate and nuclei were stained with DAPI.
Featured Resources Many resources and specialized products for researchers are available, from Application Notes to open-access tools and protocols. See the comparison table at right for label equivalents, or consult the iFluor™ Dye Selection Guide.
IHC Detection & Amplification:
Recently Added To Catalog
BSA-Compatible ReadiLink™ xtra Rapid Antibody Labeling Kits:
Online Fluorescence Spectrum Viewer
AAT Bioquest's Interactive Spectrum Viewer is a powerful tool for analysis and comparison of the spectra of common compounds used in absorbance and fluorescence formats.
If You Use: Alexa Fluor® 350, AMCA, coumarin
Try The Equivalent: iFluor™ 350
If You Use: HiLyte™ 488, Cy2, FITC
Try The Equivalent: iFluor™ 488
If You Use: Alexa Fluor® 555, HiLyte™ 555, Cy3, TRITC
Try The Equivalent: iFluor™ 555
If You Use: Alexa Fluor® 568, Atto 565, Rhodamine Red
Try The Equivalent:iFluor™ 568
If You Use: Alexa Fluor® 594, Atto 594, Texas Red
Try The Equivalent: iFluor™ 594
If You Use: Alexa Fluor® 647, Atto 647, HiLyte™ 647, Cy5
Try The Equivalent: iFluor™ 647
If You Use: Alexa Fluor® 680, Atto 680 Cy5.5, IRDye® 680
Try The Equivalent: iFluor™ 680
If You Use:
Alexa Fluor® 750, HiLyte™ 750, Cy7
Try The Equivalent:iFluor™ 750
Alexa Fluor® is a registered trademark of ThermoFisher. HiLyte™ is a trademark of AnaSpec.
Comments