Similar to A-23187, 4-Bromo A-23187 is used for in situ calibrations of fluorescent Ca² indicators to equilibrate intracellular and extracellular calcium concentrations and to allow Mn² to enter cells to quench indicator fluorescence. However, since 4-bromo A-23187 is nonfluorescent, it is preferred over the blue fluorescent A-23187 for calibrations of UV-excited Ca² indicators such as Indo-1 and Fura-2 in order to keep background fluorescence to a minimum.
<ul>
<li>White solid soluble in DMSO or EtOH</li>
<li>Store at 4°C</li>
<li>C₂₉H₃₆BrN₃O₆</li>
<li>MW: 602.52</li>
<li>[76455-48-6]</li>
</ul>