5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate. <ul> <li>&lamba;_Ex/&lamba;_Em = 495/519 nm</li> <li>Yellow solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C₂₅H₁₅NO₉</li> <li>MW: 473.4</li> <li>[117548-22-8]</li> </ul>