BCIP, toluidine (5-Bromo-4-chloro-3-indoxyl phosphate, <i>p-</i>toluidine salt) is a widely used chromogenic phosphatase substrate, which forms a dark blue (&lambda;_max 615 nm) precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride), which facilitates the precipitation.
<ul> <li>Store at 4&deg;C and protect from light</li> <li>C₁₅H₁₅BrClN₂O₄P</li> <li>MW: 433.6</li> <li>[6578-06-9]</li></ul>