Fluorescent biocytin conjugates are cell-impermeant, fixable polar tracers with biotin and an aldehyde-fixable primary amine groups.  Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has excitation/emission at 490/515 nm.