CF® dye tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF®532 dye has excitation/emission at 527/558 nm.