Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. The aequorin complex has been used as an excellent calcium indicator that can measure a broad concentration range of calcium from <em>~</em>0.1 uM to &gt;100 uM. Renilla luciferase has been widely used as a reporter protein. Renilla luciferase/coelenterazine has also been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Other uses of coelenterazine include chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues. Also see water soluble Aquaphile&#x2122; Coelenterazine.<ul> <li>Yellow solid soluble in MeOH or EtOH</li> <li>Store at -20&deg;C and protect from light</li> <li>C₂₆H₂₁N₃O₃</li> <li>MW: 423</li> <li>[55779-48-1]</li></ul>