DAPI (4',6-Diamidino-2-Phenylindole) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Because of the potential toxicity of DAPI, we offer DAPI dilactate dissolved in H₂O as a safer alternative to weighing out the powdery form. We also offer DAPI dilactate solid, which is more water soluble than the dihydrochloride form of the dye.
<ul>
<li>Classic blue nuclear counterstain for fixed cells</li>
<li>Can be used at higher concentrations to stain live cells</li>
<li>&lamba;_Ex/&lamba;_Em(with DNA) = 358/461 nm</li>
<li>Yellow solution</li>
<li>Store at 4°C and protect from light</li>
<li>C₂₂H₂₇N₅O₆</li>
<li>MW: 457.49</li>
</ul>