5(6)-Carboxytetramethylrhodamine maleimide has C6-linker that enhances the fluorescence after the dye is conjugated to proteins. The product is a direct replacement for 5(6)-carboxytetramethylrhodamine C5-maleimide (91040) <ul> <li>&lamba;_Ex/&lamba;_Em (MeOH) = 540/565 nm</li> <li>Red solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from light, especially in solution</li> <li>C₃₅H₃₆N₄O₆</li> <li>MW: 608.66</li> </ul>

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.
Key advantages of these calcium chelators include relative insensitivity toward intracellular pH change and fast release of calcium. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 offers several BAPTA chelators with calcium dissociation constants covering the biologically significant range from 10^-7 to 10^-2 M.
<ul>
<li>Kd (no Mg²): 3.6 uM</li>
<li>White to off-white solid soluble in water</li>
<li>Store at 4°C</li>
<li>C₂₂H₁₈Br₂K₄N₂O₁₀</li>
<li>MW: 786.59</li>
<li>[73630-11-2]</li>
</ul>

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.
Key advantages of these calcium chelators include relative insensitivity toward intracellular pH change and fast release of calcium. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 offers several BAPTA chelators with calcium dissociation constants covering the biologically significant range from 10^-7 to 10^-2 M.

Difluoro BAPTA AM ester is a membrane permeable form of Difluoro BAPTA that can be loaded into cells by incubation. Difluoro BAPTA AM ester itself does not bind calcium, but once inside the cell is converted into Difluoro BAPTA by cytoplasmic esterases.
<ul>
<li>Colorless solid soluble in DMSO</li>
<li>Store desiccated at -20°C</li>
<li>C₃₄H₃₈F₂N₂O₁₈</li>
<li>MW: 801</li>
<li>[156027-00-8]</li>
</ul>

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.
Key advantages of these calcium chelators include relative insensitivity toward intracellular pH change and fast release of calcium. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 offers several BAPTA chelators with calcium dissociation constants covering the biologically significant range from 10^-7 to 10^-2 M.
<ul>
<li>Kd (no Mg²): 0.59 uM, Kd (1 mM Mg²): 0.70 uM</li>
<li>White solid soluble in water</li>
<li>Store at 4°C</li>
<li>C₂₂H₁₈F₂K₄N₂O₁₀</li>
<li>MW: 665</li>
<li>[152290-47-6]</li>
</ul>

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium. Key advantages of these calcium chelators include relative insensitivity toward intracellular pH change and fast release of calcium. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 offers several BAPTA chelators with calcium dissociation constants covering the biologically significant range from 10^-7 to 10^-2 M.

Dimethyl BAPTA AM ester is a membrane permeable form of Dimethyl BAPTA that can be loaded into cells by incubation. Dimethyl BAPTA AM ester itself does not bind calcium, but once inside the cell is converted into Dimethyl BAPTA by cytoplasmic esterases.
<ul>
<li>White to off-white solid soluble in DMSO</li>
<li>Store desiccated at -20°C</li>
<li>C₃₆H₄₄N₂O₁₈</li>
<li>MW: 792.75</li>
<li>[147504-94-7]</li>
</ul>

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.

Key advantages of these calcium chelators include relative insensitivity toward intracellular pH change and fast release of calcium. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 offers several BAPTA chelators with calcium dissociation constants covering the biologically significant range from 10^-7 to 10^-2 M.
<ul>
<li>Kd (no Mg²): 0.16 uM, Kd (1 mM Mg²): 0.44 uM</li>
<li>White solid soluble in water</li>
<li>Store at -20°C</li>
<li>C₂₄H₂₄K₄N₂O₁₀</li>
<li>MW: 657</li>
<li>[73630-09-8]</li>
</ul>

5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)-carboxy-2',7'-dichlorofluorescein by intracellular esterases.
<ul>
<li>White solid soluble in DMSO</li>
<li>Store at 4°C and protect from light, especially in solution</li>
<li>C₂₅H₁₄Cl₂O₉</li>
<li>MW: 529</li>
<li>[127770-45-0]</li>
</ul>

5-(and-6)-carboxy-2',7'-dichlorofluorescein is similar to carboxyfluorescein, but has lower pK_a. It is useful for detecting pH in more acidic environment. Also see the membrane-permeant compound dichlorofluorescein diacetate.
<ul>
<li>pK_a = 4.8</li>
<li>&lamba;_Ex/&lamba;_Em = 504/529 nm</li>
<li>&epsilon;= 90,000</li>
<li>Orange solid soluble in DMSO and pH&gt;6 buffers</li>
<li>Store at room temperature and protect from light, especially in solution</li>
<li>C₂₁H₁₀Cl₂O₇</li>
<li>MW: 445</li>
<li>[111843-78-8]</li>
</ul>

5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases. The fluorescence of the dye is relatively insensitive to pH change and the labeling has been shown to be stable to formaldehyde or gluteraldehyde fixation. <ul> <li>&lamba;_Ex/&lamba;_Em(of hydrolysis product at pH 7) = 504/529 nm</li> <li>White solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C₂₉H₁₇Cl₂NO₁₁</li> <li>MW: 626</li> </ul>

5(6)-ROX, SE (full name: 5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester) is an amine-reactive long wavelength rhodamine dye. <ul> <li>&lamba;_Ex/&lamba; _Em (MeOH) = 568/595 nm</li> <li>Dark red solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C₃₇H₃₃N₃O₇</li> <li>MW: 631.7</li> <li>[114616-32-9]</li> </ul>

5(6)-ROX (full name: 5-(and-6)-Carboxy-X-rhodamine, triethylammonium salt (mixed isomers)) is a long wavelength rhodamine dye.
<ul>
<li>&lamba;_Ex/&lamba;_Em (MeOH) = 568/595 nm</li>
<li>Dark red solid soluble in DMSO, DMF or MeOH</li>
<li>Store at -20°C and protect from light</li>
<li>C₃₉H₄₅N₃O₅</li>
<li>MW: 635.6</li>
</ul>

For some applications, 5-(and-6)-carboxyrhodamine (5(6)-CR 110) is a superior alternative to the widely used green fluorescent dye 5-(and-6)-carboxyfluorescein. The dye is insensitive to pH between 4 and 9 and is much more photostable than fluorescein.

<ul>
<li>&lamba;_Ex/&lamba;_Em (MeOH) = 502/524 nm</li>
<li>Orange red solid soluble in H₂O (pH&gt;6), DMF or DMSO</li>
<li>Store at 4°C and protect from light</li>
<li>C₂₁H₁₅ClN₂O₅</li>
<li>MW: 410.81</li>
</ul>

5-(and-6)-Carboxyrhodamine 110, SE (5-(and-6)-Carboxyrhodamine 110, succinimidyl ester, mixed isomers), also known as Carboxyrhodamine 110-NHS, is a superior green fluorescent dye that readily reacts with primary or secondary amines. For many applications, the dye is preferred over 5-(and-6)-carboxyfluorescein succinimidyl ester (5(6)-FAM) or FITC because of its exceptional photostability and fluorescence insensitivity to pH between 4-9.
<ul>
<li>&lamba;_Ex/&lamba; _Em(MeOH) = 502/524 nm</li>
<li>Orange red solid soluble in DMF or DMSO</li>
<li>Store at -20°C and protect from light</li>
<li>C₂₅H₁₇N₃O₇</li>
<li>MW:471.42</li>
</ul>

5-(and-6)-Carboxyrhodamine 110-X (full name: 5-(and-6)-Carboxyrhodamine 110-X, hydrochloride) is similar to 5-(and-6)-carboxyrhodamine 110. The X linker molecule is useful to reduce the interaction between the dye and the molecule to be labeled.

<ul>

<li>&lamba;_Ex/&lamba;_Em (MeOH) = 502/524 nm</li>

<li>Orange red solid soluble in water, DMF or DMSO</li>

<li>Store at 4°C and protect from light</li>

<li>C₂₈H₃₀ClN₃O₆</li>

<li>MW:524</li>

</ul>

5-(and-6)-Carboxyrhodamine 110-X, succinimidyl ester (5(6)-CR 110-X, SE) readily couples to a primary or secondary amine. The dye is a superior alternative to 5(6)-SFX because of its exceptional phototostability and fluorescence insensitivity to pH between 4-9.
<ul>
<li>&lamba;_Ex/&lamba; _Em (MeOH) = 502/524 nm</li>
<li>Orange red solid soluble in DMSO or DMF.</li>
<li>Store at -20°C and protect from light</li>
<li>C₃₁H₂₈N₄O₈</li>
<li>MW: 584.58</li>
</ul>