5(6)-TAMRA SE (full name: 5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester (mixed isomers)) is the amine reactive form of TAMRA, one of the most commonly used red fluorescent dyes. The succinimidyl ester reacts readily with primary or secondary amines under mild conditions.

<ul>

<li>&lamba;_Ex/&lamba;_Em (MeOH) = 540/565 nm</li>

<li>&epsilon; = 95,000</li>

<li>Red solid soluble in DMF or DMSO</li>

<li>Store at -20°C and protect from light</li>

<li>C₂₉H₂₅N₃O₇</li>

<li>MW: 527.5</li>

<li>[150408-83-6]</li>

</ul>

Mixed isomers of carboxytetramethylrhodamine (TAMRA) free acid. The dye can be coupled to primary or secondary amines via standard peptide chemistry.

<ul>

<li>&lambda;_Ex/&lambda;_Em (MeOH) = 540/565 nm</li>

<li>Dark red solid soluble in DMSO, DMF, MeOH, or pH &gt;6.5 buffer</li>

<li>Store at -20&deg;C and protect from light</li>

<li>C₂₅H₂₃ClN₂O₅</li>

<li>MW: 466.92</li>

</ul>

5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator. Its excitation spectrum and fluorescence response to pH are similar to those of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.
<ul>
<li>&lamba;_Ex/&lamba;_Em (pH 9) = 492/514 nm</li>
<li>&epsilon; (pH 9) = 82,000</li>
<li>Yellow orange solid soluble in pH&gt;6 water</li>
<li>Store at 4°C and protect from light, especially in solution</li>
<li>C₂₁H₁₂O₇</li>
<li>MW: 376</li>
<li>[72088-94-9]</li>
</ul>

5-(and 6-)Carboxyrhodamine 6G, hydrochloride, mixed isomers is the free acid form of orange fluorescent carboxyrhodamine 6G dye (Ex/Em 520/546 nm). Unit size: 5 mg.

INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins.
<ul>
<li>Light yellow solid soluble in DMSO or DMF</li>
<li>Store at 4°C and protect from light</li>
<li>C₁₀H₆IN₃</li>
<li>MW: 295.08</li>
</ul>

83% 5-APB

83% 5-APB

83% 5-APB

100% 5-APB

5-Aminoallyl-dUTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled dUTP.
<ul>
<li>White solid soluble in water</li>
<li>Store at -20°C</li>
<li>C₁₂H₁₇N₃Na₃O₁₄P₃</li>
<li>MW: 589.17</li>
<li>&ge;95% by HPLC at 290 nm</li>
</ul>
AA-dUTP is also available as a 10 mM solution in TE buffer.

5-Aminoallyl-UTP, sodium salt, lyophilized powder; alternate name(s): 5-(3-Aminoallyl)uridine-5'-triphosphate, trisodium salt; AA-UTP

5-Aminoallyl-UTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling methods.
<ul>
<li>Store at -20°C</li>
<li>C₁₂H₁₇N₃Na₃O₁₅P₃</li>
<li>MW: 605.17</li>
</ul>
9689c5aa-6556-4b3c-b5d1-1e693715ba07 also offers 5-Aminoallyl-UTP at 10 mM in TE buffer.

5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled dUTP.
<ul>
<li>10 mM solution in TE buffer</li>
<li>Store at -20°C</li>
<li>C₁₂H₁₇N₃Na₃O₁₄P₃</li>
<li>MW: 589.17</li>
</ul>
AA-dUTP is also available in lyophilized form.

5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled dUTP.
<ul>
<li>10 mM solution in TE buffer</li>
<li>Store at -20°C</li>
<li>C₁₂H₁₇N₃Na₃O₁₄P₃</li>
<li>MW: 589.17</li>
</ul>
AA-dUTP is also available in lyophilized form.

BrdU (5-Bromo-2'-deoxyuridine) can be incorporated into DNA during cell division and subsequently detected by a BrdU a4d73786-81ea-96bb-77d4-9ff261b5ff15. The probe can be used to study cell-cycle kinetics.
<ul>
<li>White solid soluble in DMSO</li>
<li>Store at -20°C</li>
<li>C₉H₁₁BrN₂O₅</li>
<li>MW: 307.10</li>
<li>[59-14-3]</li>
</ul>

5-Bromo-dUTP (5-Bromo-2'-deoxyuridine-5'-triphosphate) is widely used in TUNEL assay to detect apoptotic cells, and it is also a good substrate for reverse transcriptase.
<ul>
<li>10 mM solution in TE buffer</li>
<li>Store at -20°C</li>
<li>C₉H₁₁BrN₂Na₃O₁₄P₃</li>
<li>MW: 612.99</li>
</ul>