Description & Target

Aminooxy-5(6)-ROX reacts with aldehydes or ketones to a stable hydrazone compound under mild conditions. Similar to ARP, aminooxy-5(6)-ROX can be used to label abasic sites in damaged DNA. <ul> <li>&lamba;_Ex/&lamba;_Em (MeOH): 568/595 nm</li> <li>Dark purple solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from moisture and light</li> <li>C₄₅H₅₁F₆N₅O₁₀</li> <li>MW: 935.90</li> </ul>

Description & Target

Aminooxy-5(6)-ROX reacts with aldehydes or ketones to form a stable hydrazone compound under mild condition. Similar to ARP, aminooxy-5(6)-TAMRA can be used to label abasic sites in damaged DNA. <ul> <li>&lamba;_Ex/&lamba; _Em(MeOH) = 540/565 nm</li> <li>Dark red solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from moisture and light</li> <li>C₃₆H₃₉F₆N₅O₁₀</li> <li>MW: 815.71</li> </ul>

Description & Target

Aminooxy-biotin can be used to covalently attach biotin to a molecule with an aldehyde or ketone group. The aminooxy group reacts with an aldehyde or ketone group to form a stable oxime linkage in an aqueous solution at neutral pH. The reaction is fast and can be further accelerated by adding aniline as a catalyst. Thus, aminooxy reagents are superior to hydrazide reagents, which also react with aldehydes or ketones but form unstable hydrazone linkages.

Description & Target

Aniline, 10X in acetate buffer is designed to use as catalytic reagent for the optimal coupling of protein carbonyls with aminooxy compounds.

Description & Target

Annexin V conjugated to Allophycocyanin (APC) can be used to detect apoptotic cells by flow cytometry. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.

Description & Target

Annexin V conjugated to Allophycocyanin (APC) can be used to detect apoptotic cells by flow cytometry. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.

Description & Target

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V. Biotin Annexin V conjugate is provided at 50 ug/mL in buffer with azide as a preservative.

Description & Target

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V. CF&reg; Dye Annexin V Conjugates are provided at 50 ug/mL in buffer with azide as a preservative. CF&reg; dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. UV-excitable blue fluorescent CF&reg;350 dye has excitation/emission at 347/448 nm.

Description & Target

CF®350 Annexin V is a UV-excitable blue fluorescent conjugate (Ex/Em 347/448 nm) that can be used to stain phosphatidylserine on the surface of apoptotic cells. This Annexin V conjugate is supplied lyophilized and azide-free, compatible with real-time imaging of live cells. Unit size 5 ug.

Description & Target

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V. CF&reg; Dye Annexin V Conjugates are provided at 50 ug/mL in buffer with azide as a preservative. CF&reg; dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF&reg;405M dye has excitation/emission at 408/452 nm.

Description & Target

CF®405M Annexin V is a blue fluorescent conjugate (Ex/Em 408/452 nm) that can be used to stain phosphatidylserine on the surface of apoptotic cells. This Annexin V conjugate is supplied lyophilized and azide-free, compatible with real-time imaging of live cells. Unit size 5 ug.

Description & Target

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V. CF&reg; Dye Annexin V Conjugates are provided at 50 ug/mL in buffer with azide as a preservative. CF&reg; dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. 405 nm-excitable green fluorescent CF&reg;450 dye has excitation/emission at 450/538 nm.

Description & Target

CF®450 Annexin V is a photostable violet-excitable green fluorescent conjugate (Ex/Em 405/460 nm) that can be used to stain phosphatidylserine on the surface of apoptotic cells. It is supplied lyophilized and azide-free, compatible with real-time imaging of live cells. Unit size 5 ug.

Description & Target

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V. CF&reg; Dye Annexin V Conjugates are provided at 50 ug/mL in buffer with azide as a preservative. CF&reg; dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF&reg;488A dye has excitation/emission at 490/515 nm.

Description & Target

CF®488A Annexin V is a green fluorescent conjugate (Ex/Em 490/515 nm) that can be used to stain phosphatidylserine on the surface of apoptotic cells. This Annexin V conjugate is supplied lyophilized and azide-free, compatible with real-time imaging of live cells. Unit size 5 ug.