Description & Target

5-(and-6)-carboxy-2',7'-dichlorofluorescein is similar to carboxyfluorescein, but has lower pK_a. It is useful for detecting pH in more acidic environment. Also see the membrane-permeant compound dichlorofluorescein diacetate.
<ul>
<li>pK_a = 4.8</li>
<li>&lamba;_Ex/&lamba;_Em = 504/529 nm</li>
<li>&epsilon;= 90,000</li>
<li>Orange solid soluble in DMSO and pH&gt;6 buffers</li>
<li>Store at room temperature and protect from light, especially in solution</li>
<li>C₂₁H₁₀Cl₂O₇</li>
<li>MW: 445</li>
<li>[111843-78-8]</li>
</ul>

Description & Target

5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases. The fluorescence of the dye is relatively insensitive to pH change and the labeling has been shown to be stable to formaldehyde or gluteraldehyde fixation. <ul> <li>&lamba;_Ex/&lamba;_Em(of hydrolysis product at pH 7) = 504/529 nm</li> <li>White solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C₂₉H₁₇Cl₂NO₁₁</li> <li>MW: 626</li> </ul>

Description & Target

5(6)-ROX, SE (full name: 5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester) is an amine-reactive long wavelength rhodamine dye. <ul> <li>&lamba;_Ex/&lamba; _Em (MeOH) = 568/595 nm</li> <li>Dark red solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C₃₇H₃₃N₃O₇</li> <li>MW: 631.7</li> <li>[114616-32-9]</li> </ul>

Description & Target

5(6)-ROX (full name: 5-(and-6)-Carboxy-X-rhodamine, triethylammonium salt (mixed isomers)) is a long wavelength rhodamine dye.
<ul>
<li>&lamba;_Ex/&lamba;_Em (MeOH) = 568/595 nm</li>
<li>Dark red solid soluble in DMSO, DMF or MeOH</li>
<li>Store at -20°C and protect from light</li>
<li>C₃₉H₄₅N₃O₅</li>
<li>MW: 635.6</li>
</ul>

Description & Target

For some applications, 5-(and-6)-carboxyrhodamine (5(6)-CR 110) is a superior alternative to the widely used green fluorescent dye 5-(and-6)-carboxyfluorescein. The dye is insensitive to pH between 4 and 9 and is much more photostable than fluorescein.

<ul>
<li>&lamba;_Ex/&lamba;_Em (MeOH) = 502/524 nm</li>
<li>Orange red solid soluble in H₂O (pH&gt;6), DMF or DMSO</li>
<li>Store at 4°C and protect from light</li>
<li>C₂₁H₁₅ClN₂O₅</li>
<li>MW: 410.81</li>
</ul>

Description & Target

5-(and-6)-Carboxyrhodamine 110, SE (5-(and-6)-Carboxyrhodamine 110, succinimidyl ester, mixed isomers), also known as Carboxyrhodamine 110-NHS, is a superior green fluorescent dye that readily reacts with primary or secondary amines. For many applications, the dye is preferred over 5-(and-6)-carboxyfluorescein succinimidyl ester (5(6)-FAM) or FITC because of its exceptional photostability and fluorescence insensitivity to pH between 4-9.
<ul>
<li>&lamba;_Ex/&lamba; _Em(MeOH) = 502/524 nm</li>
<li>Orange red solid soluble in DMF or DMSO</li>
<li>Store at -20°C and protect from light</li>
<li>C₂₅H₁₇N₃O₇</li>
<li>MW:471.42</li>
</ul>

Description & Target

5-(and-6)-Carboxyrhodamine 110-X (full name: 5-(and-6)-Carboxyrhodamine 110-X, hydrochloride) is similar to 5-(and-6)-carboxyrhodamine 110. The X linker molecule is useful to reduce the interaction between the dye and the molecule to be labeled.

<ul>

<li>&lamba;_Ex/&lamba;_Em (MeOH) = 502/524 nm</li>

<li>Orange red solid soluble in water, DMF or DMSO</li>

<li>Store at 4°C and protect from light</li>

<li>C₂₈H₃₀ClN₃O₆</li>

<li>MW:524</li>

</ul>

Description & Target

5-(and-6)-Carboxyrhodamine 110-X, succinimidyl ester (5(6)-CR 110-X, SE) readily couples to a primary or secondary amine. The dye is a superior alternative to 5(6)-SFX because of its exceptional phototostability and fluorescence insensitivity to pH between 4-9.
<ul>
<li>&lamba;_Ex/&lamba; _Em (MeOH) = 502/524 nm</li>
<li>Orange red solid soluble in DMSO or DMF.</li>
<li>Store at -20°C and protect from light</li>
<li>C₃₁H₂₈N₄O₈</li>
<li>MW: 584.58</li>
</ul>

Description & Target

5(6)-TAMRA SE (full name: 5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester (mixed isomers)) is the amine reactive form of TAMRA, one of the most commonly used red fluorescent dyes. The succinimidyl ester reacts readily with primary or secondary amines under mild conditions.

<ul>

<li>&lamba;_Ex/&lamba;_Em (MeOH) = 540/565 nm</li>

<li>&epsilon; = 95,000</li>

<li>Red solid soluble in DMF or DMSO</li>

<li>Store at -20°C and protect from light</li>

<li>C₂₉H₂₅N₃O₇</li>

<li>MW: 527.5</li>

<li>[150408-83-6]</li>

</ul>

Description & Target

Mixed isomers of carboxytetramethylrhodamine (TAMRA) free acid. The dye can be coupled to primary or secondary amines via standard peptide chemistry.

<ul>

<li>&lambda;_Ex/&lambda;_Em (MeOH) = 540/565 nm</li>

<li>Dark red solid soluble in DMSO, DMF, MeOH, or pH &gt;6.5 buffer</li>

<li>Store at -20&deg;C and protect from light</li>

<li>C₂₅H₂₃ClN₂O₅</li>

<li>MW: 466.92</li>

</ul>

Description & Target

5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator. Its excitation spectrum and fluorescence response to pH are similar to those of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.
<ul>
<li>&lamba;_Ex/&lamba;_Em (pH 9) = 492/514 nm</li>
<li>&epsilon; (pH 9) = 82,000</li>
<li>Yellow orange solid soluble in pH&gt;6 water</li>
<li>Store at 4°C and protect from light, especially in solution</li>
<li>C₂₁H₁₂O₇</li>
<li>MW: 376</li>
<li>[72088-94-9]</li>
</ul>

Description & Target

5-(and 6-)Carboxyrhodamine 6G, hydrochloride, mixed isomers is the free acid form of orange fluorescent carboxyrhodamine 6G dye (Ex/Em 520/546 nm). Unit size: 5 mg.

Description & Target

INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins.
<ul>
<li>Light yellow solid soluble in DMSO or DMF</li>
<li>Store at 4°C and protect from light</li>
<li>C₁₀H₆IN₃</li>
<li>MW: 295.08</li>
</ul>

Description & Target

5-Aminoallyl-dUTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled dUTP.
<ul>
<li>White solid soluble in water</li>
<li>Store at -20°C</li>
<li>C₁₂H₁₇N₃Na₃O₁₄P₃</li>
<li>MW: 589.17</li>
<li>&ge;95% by HPLC at 290 nm</li>
</ul>
AA-dUTP is also available as a 10 mM solution in TE buffer.

Description & Target

5-Aminoallyl-UTP, sodium salt, lyophilized powder; alternate name(s): 5-(3-Aminoallyl)uridine-5'-triphosphate, trisodium salt; AA-UTP

5-Aminoallyl-UTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling methods.
<ul>
<li>Store at -20°C</li>
<li>C₁₂H₁₇N₃Na₃O₁₅P₃</li>
<li>MW: 605.17</li>
</ul>
9689c5aa-6556-4b3c-b5d1-1e693715ba07 also offers 5-Aminoallyl-UTP at 10 mM in TE buffer.