Description & Target

5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled dUTP.
<ul>
<li>10 mM solution in TE buffer</li>
<li>Store at -20°C</li>
<li>C₁₂H₁₇N₃Na₃O₁₄P₃</li>
<li>MW: 589.17</li>
</ul>
AA-dUTP is also available in lyophilized form.

Description & Target

5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled dUTP.
<ul>
<li>10 mM solution in TE buffer</li>
<li>Store at -20°C</li>
<li>C₁₂H₁₇N₃Na₃O₁₄P₃</li>
<li>MW: 589.17</li>
</ul>
AA-dUTP is also available in lyophilized form.

Description & Target

BrdU (5-Bromo-2'-deoxyuridine) can be incorporated into DNA during cell division and subsequently detected by a BrdU a4d73786-81ea-96bb-77d4-9ff261b5ff15. The probe can be used to study cell-cycle kinetics.
<ul>
<li>White solid soluble in DMSO</li>
<li>Store at -20°C</li>
<li>C₉H₁₁BrN₂O₅</li>
<li>MW: 307.10</li>
<li>[59-14-3]</li>
</ul>

Description & Target

5-Bromo-dUTP (5-Bromo-2'-deoxyuridine-5'-triphosphate) is widely used in TUNEL assay to detect apoptotic cells, and it is also a good substrate for reverse transcriptase.
<ul>
<li>10 mM solution in TE buffer</li>
<li>Store at -20°C</li>
<li>C₉H₁₁BrN₂Na₃O₁₄P₃</li>
<li>MW: 612.99</li>
</ul>

Description & Target

BrUTP (5-Bromouridine-5'-triphosphate) can be enzymatically incorporated into RNA and detected with anti-BrdU antibodies.
<ul>
<li>10 mM solution in TE buffer</li>
<li>Store at -20°C</li>
<li>C₉H₁₁BrN₂Na₃O₁₅P₃</li>
<li>MW: 628.98</li>
</ul>

Description & Target

5-ROX, SE (5-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 5-carboxy-X-rhodamine single isomer. <ul> <li>&lamba;_Ex/&lamba; _Em(MeOH) = 568/595 nm</li> <li>Dark red solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C₃₇H₃₃N₃O₇</li> <li>MW: 631.7</li> <li>[344402-35-3]</li> </ul>

Description & Target

5-ROX (full name: 5-Carboxy-X-rhodamine, triethylammonium salt, single isomer) is a single isomer, long wavelength rhodamine dye.
<ul>
<li>&lamba;_Ex/&lamba;_Em(MeOH) = 568/595 nm</li>
<li>Dark red solid soluble in DMSO, DMF or MeOH</li>
<li>Store at 4°C and protect from light</li>
<li>C₃₉H₄₅N₃O₅</li>
<li>MW: 635.6</li>
</ul>

Description & Target

Amine-reactive form of 5-carboxyfluorescein single isomer. <ul> <li>&lamba;_Ex/&lamba; _Em(pH 9) = 495/519 nm</li> <li>Yellow orange solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from light</li> <li>C₂₅H₁₅NO₉</li> <li>MW: 473.4</li> <li>[92557-80-7]</li> </ul>

Description & Target

Amine-reactive form of single isomer carboxyrhodamine 110, useful in DNA sequencing. For many single-dye-labeling applications, the dye is preferred over other green fluorescent dyes including fluorescein and Alexa 488 because of its exceptional photostability and fluorescence insensitivity to pH (4-9). <ul> <li>&lamba;_Ex/&lamba; _Em(MeOH) = 502/524 nm</li> <li>Orange red solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from light</li> <li>C₃₃H₃₆N₄O₇</li> <li>MW: 600.67</li> </ul>

Description & Target

5-CR6G (full name: 5-Carboxyrhodamine 6G, hydrochloride, single isomer) is the free acid form of 5-carboxyrhodamine 6G single isomer.
<ul>
<li>&lamba;_Ex/&lamba; _Em(MeOH) = 520/546 nm</li>
<li>Orange red solid soluble in water (pH 7), DMF or DMSO</li>
<li>Store at 4°C and protect from light</li>
<li>C₂₇H₂₇ClN₂O₅</li>
<li>MW: 494.97</li>

Description & Target

Amine-reactive form of 5-carboxyrhodamine 6G single isomer. <ul> <li>&lamba;_Ex/&lamba; _Em(MeOH) = 520/546 nm</li> <li>Orange red solid moderately soluble in DMSO</li> <li>Store at -20°C and protect from light</li> <li>C₃₁H₂₉ClN₃O₇</li> <li>MW: 555.6</li> </ul>

Description & Target

Full name: 5-Carboxytetramethylrhodamine succinimidyl ester (single isomer). Amine-reactive form of carboxytetramethylrhodamine (TAMRA) single isomer. <ul> <li>&lamba;_Ex/&lamba;_Em (MeOH) = 540/565 nm</li> <li>Dark red solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C₂₉H₂₅N₃O₇</li> <li>MW: 527.5</li> <li>[150810-68-7]</li> </ul>

Description & Target

Free acid form of 5-TAMRA (5-Carboxytetramethylrhodamine, single isomer).
<ul>
<li>&lamba;_Ex/&lamba;_Em (MeOH) = 540/565 nm</li>
<li>Dark red solid soluble in DMSO, DMF, or MeOH</li>
<li>C₂₅H₂₂N₂O₅</li>
<li>MW: 430.5</li>
<li>[91809-66-4]</li>

Description & Target

5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein.
<ul>
<li>Off-white solid soluble in DMSO</li>
<li>Store desiccated at 4°C</li>
<li>C₂₅H₁₆O₉</li>
<li>MW: 460</li>
<li>[79955-27-4]</li>
</ul>

Description & Target

5-CR110-PEO₃-amine has a long water-soluble PEG spacer that separates the green fluorescent CR110 and the amine functional group. <ul> <li>&lamba;_Ex/&lamba;_Em(MeOH) = 502/524 nm</li> <li>Orange yellow solid soluble in DMSO, DMF, or H2O</li> <li>Store at -20°C and protect from light</li> <li>C₃₃H₃₄F₆N₄O₁₁</li> <li>MW: 776.63</li> </ul>