Description & Target

5-CR110-PEO₄ SE (full name: 5-Carboxyrhodamine 110-PEO₄-propionate, succinimidyl ester, single isomer) is an excellent green fluorescent rhodamine dye for labeling amines. The dye has better solubility than the parent dye 5-CR110 SE and its long PEG spacer should reduce any steric effect of the dye on the molecule to be labeled. Carboxyrhodamine 110 and its derivatives are, in general, significantly more photostable than any other known green fluorescent dyes including Alexa Fluor&reg; 488. In addition, their spectra are unaffected by pH change from 4 to 9. Unlike Alexa Fluor&reg; 488, which is readily degraded under alkaline conditions, CR110 and its derivatives are highly stable under both acidic and basic conditions. As a result, CR110 and its derivatives are superior alternatives to other green fluorescent dyes for applications where only a single dye is required for the labeling. <ul> <li>&lamba;_Ex/&lamba;_Em(MeOH) = 502/524 nm</li> <li>Orange red solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from light</li> <li>C₃₆H₃₈N₄O₁₂</li> <li>MW: 718.71</li> </ul>

Alexa Fluor is a registered trademark of Thermo Fisher Scientific.

Description & Target

5-CR110-X, SE (full name: 5-Carboxyrhodamine 110-X, succinimidyl ester) is the amine-reactive form of 5-carboxyrhodamine 110 with a 6-carbon spacer. <ul> <li>&lamba;_Ex/&lamba; _Em(MeOH) = 502/524 nm</li> <li>Orange red solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C₃₁H₂₈N₄O₈</li> <li>MW: 584.58</li> </ul>

Description & Target

This dye can be used as a low pH indicator similar 5-and-6-Carboxy-2'7'-dichlorofluorescein, and is also useful as a general green fluorescent label. Carboxy-2',7'-dichlorofluorescein is significantly more photostable than carboxyfluorescein.
<ul>
<li>&lamba;_Ex/&lamba;_Em = 504/529 nm (pH 8)</li>
<li>&epsilon; = 90,000</li>
<li>Yellow solid soluble in DMSO, DMF or basic water (pH &gt;7)</li>
<li>Store at  4°C and protect from light</li>
<li>C₂₁H₁₀Cl₂O₇</li>
<li>MW: 445</li>
</ul>

Description & Target

The pKa of 5-carboxy-2',7'-dichlorosulfonefluorescein is near 4 and thus the dye is potentially an excellent pH indicator for acidic organelles.
<ul>
<li>&lamba;_Ex/&lamba; _Em= 509/532nm (pH 9)</li>
<li>&epsilon; = 92,000</li>
<li>Yellow solid soluble in DMSO, DMF or a buffer (pH &gt;6)</li>
<li>Store at 4°C and protect from light</li>
<li>C₂₀H₁₀Cl₂O₈S</li>
<li>MW: 481.26</li>
</ul>

Description & Target

5-ROX-PEO₈ SE (full name: 5-Carboxy-X-rhodamine-PEO₈-propionate, succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates the red fluorescent ROX dye and the amine-reactive SE group. <ul> <li>&lamba;_Ex/&lamba; _Em(MeOH) = 568/595 nm</li> <li>Dark red solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C₅₆H₇₀N₄O₁₆</li> <li>MW: 1055.17</li> </ul>

Description & Target

5-FAM (full name: 5-Carboxyfluorescein, single isomer) is the free acid form of 5-carboxyfluorescein single isomer.
<ul>
<li>&lamba;Ex/&lambda;Em (pH 9) = 492/514 nm</li>
<li>Yellow orange solid soluble in pH&gt;6 water</li>
<li>Store at 4&deg;C and protect from light, especially in solution</li>
<li>C₂₁H₁₂O₇</li>
<li>MW: 376.3</li>
<li>[76823-03-5]</li>
</ul>

Description & Target

5-FAM-PEO₈ SE (full name: 5-Carboxyfluorescein-PEO₈ propionate succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates fluorescein and the amine-reactive SE group. <ul> <li>&lamba;_Ex/&lamba; _Em(pH9) = 494/521 nm</li> <li>Yellow orange solid soluble in DMF, DMSO, or pH 6.5 buffer</li> <li>Store at -20°C and protect from light</li> <li>C₄₄H₅₂N₂O₁₈</li> <li>MW:896.89</li> </ul>

Description & Target

5-SFX (full name: 6-(Fluorescein-5-carboxamido)hexanoic acid, succinimidyl ester, single isomer) has a 6-atom aminohexanoyl spacer group that separates the fluorescein dye and the amine-reactive group. <ul> <li>&lamba;_Ex/&lamba; _Em(pH9) = 494/521nm</li> <li>Yellow solid soluble in DMF, DMSO or pH&gt;6.5 buffers</li> <li>Store at -20°C and protect from light</li> <li>C₃₁H₂₆N₂O₁₀</li> <li>MW:586.55</li> </ul>

Description & Target

5-TAMRA-PEO3-amine (Tetramethylrhodamine-PEO3-amine) has a long water-soluble PEG spacer separating the dye and the amine functional group. The dye can be coupled to activated carboxy groups or sulfonyl chlorides.
<ul>
<li>&lamba;_Ex/&lamba;_Em (MeOH) = 544/571 nm</li>
<li>Dark purple solid soluble in DMSO, DMF, or H2O</li>
<li>Store at 4°C and protect from light</li>
<li>C₃₇H₄₂F₆N₄O₁₁</li>
<li>MW: 832.74</li>

Description & Target

5-TAMRA-dUTP (5-Tetramethylrhodamine-dUTP) can be enzymatically incorporated into DNA. Note: 5-TAMRA-dUTP may not be compatible with PCR labeling of DNA probes. Supplied as a 1 mM solution in Tris pH 7.5 buffer.

Description & Target

Concentrated Annexin V Binding Buffer to be used for Annexin V staining.

Description & Target

5X Firefly Lysis Buffer is a concentrated cell lysis buffer that is designed for use with 9689c5aa-6556-4b3c-b5d1-1e693715ba07's Firefly Luciferase Assay Kit 2.0 and Firefly Luciferase Assay Kit (Lyophilized)</span>. These kits are supplied with sufficient 5X Firefly Lysis Buffer to perform the stated number of assays with cells grown in 96 to 24 well plates. 5X Firefly Lysis Buffer is sold separately for use with the kits when more lysis buffer (e.g. &gt;100 uL/well) is required.

Description & Target

5X Passive Lysis Buffer is concentrated lysis buffer designed for use with 9689c5aa-6556-4b3c-b5d1-1e693715ba07's Renilla Luciferase Assay Kit 2.0. Those kits are supplied with sufficient 5X Passive Lysis Buffer to complete the stated number of assays using 96-well to 24-well plates. 5X Passive Lysis Buffer is sold separately for use with the kits when more lysis buffer (e.g., &gt;100 uL per well) is required.

Description & Target

5(6)-SFX (full name: 6-(Fluorescein-5-(and-6)-carboxamido)hexanoic acid,succinimidyl ester) has a 6-atom aminohexanoyl spacer group that separates the fluorescein dye and the amine-reactive group. <ul> <li>&lamba;_Ex/&lamba; _Em(pH9) = 494/521nm</li> <li>Yellow solid soluble in DMF, DMSO or pH&gt;6.5 buffers</li> <li>Store at -20°C and protect from light</li> <li>C₃₁H₂₆N₂O₁₀</li> <li>MW:586.55</li> </ul>

Description & Target

6-ROX, SE is the amine-reactive form of 6-carboxy-X-rhodamine single isomer. <ul> <li>&lamba;_Ex/&lamba; _Em(MeOH) = 568/595 nm</li> <li>Dark red solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C₃₇H₃₃N₃O₇</li> <li>MW: 631.7</li> <li>[216699-36-4]</li> </ul>