Description & Target

6-ROX (6-Carboxy-X-rhodamine) is a single isomer, long wavelength rhodamine dye.
<ul>
<li>&lamba;_Ex/&lamba; _Em(MeOH) = 568/595 nm</li>
<li>Dark red solid soluble in DMSO, DMF or MeOH</li>
<li>Store at 4°C and protect from light</li>
<li>C₃₉H₄₅N₃O₅</li>
<li>MW: 635.6</li>

Description & Target

6-FAM, SE (full name: 6-Carboxyfluorescein, succinimidyl ester, single isomer) is the amine-reactive form of 6-carboxyfluorescein single isomer. <ul> <li>&lamba;_Ex/&lamba;_Em (pH 9) = 495/519 nm</li> <li>Yellow-orange solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from light</li> <li>C₂₅H₁₅NO₉</li> <li>MW: 473.4</li> </ul>

Description & Target

Amine-reactive form of single isomer carboxyrhodamine 110. For certain applications, the dye is preferred over the corresponding 6-carboxyfluorescein because of its exceptional photostability and fluorescence insensitivity to pH change (4-9). <ul> <li>&lamba;_Ex/&lamba; _Em(MeOH) = 502/524 nm</li> <li>Orange red solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from light</li> <li>C₃₃H₃₆N₄O₇</li> <li>MW: 600.67</li> </ul>

Description & Target

6-CR6G (full name: 6-Carboxyrhodamine 6G, hydrochloride, single isomer) is the free acid form of 6-carboxyrhodamine 6G single isomer.
<ul>
<li>&lamba;_Ex/&lamba; _Em (MeOH) = 520/546 nm</li>
<li>Orange red solid soluble in water (pH 7), DMF or DMSO</li>
<li>Store at 4°C and protect from light</li>
<li>C₂₇H₂₇ClN₂O₅</li>
<li>MW: 494.97</li>

Description & Target

Amine-reactive form of 6-carboxyrhodamine 6G single isomer. <ul> <li>&lamba;_Ex/&lamba; _Em(MeOH) = 520/546 nm</li> <li>Orange red solid moderately soluble in DMSO</li> <li>Store at -20°C and protect from light</li> <li>C₃₁H₂₉ClN₃O₇</li> <li>MW: 555.6</li> </ul>

Description & Target

Full name: 6-Carboxytetramethylrhodamine succinimidyl ester single isomer. Amine-reactive form ofcarboxytetramethylrhodamine (TAMRA) single isomer. <ul> <li>&lamba;_Ex/&lamba;_Em (MeOH) = 540/565 nm</li> <li>Dark red solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C₂₉H₂₅N₃O₇</li> <li>MW: 527.5</li> <li>[150810-69-8]</li> </ul>

Description & Target

Free acid form of 6-TAMRA (6-carboxytetramethylrhodamine) single isomer. <ul> <li>&lamba;_Ex/&lamba;_Em (MeOH) = 540/565 nm</li> <li>Dark red solid soluble in DMSO, DMF, MeOH, or pH&gt;6.5 buffers</li> <li>C₂₅H₂₂N₂O₅</li> <li>MW: 430.5</li> <li>[91809-67-5]</li> </ul>

Description & Target

6-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 6-CFDA is hydrolyzed by intracellular esterases to 6-carboxyfluorescein.
<ul>
<li>Off-white solid soluble in DMSO</li>
<li>Store desiccated at -20°C</li>
<li>C₂₅H₁₆O₉</li>
<li>MW: 460</li>
<li>[3348-03-6]</li>
</ul>

Description & Target

6-FAM (full name: 6-Carboxyfluorescein, single isomer) is the free acid form of 6-carboxyfluorescein single isomer.
<ul>
<li>&lamba;Ex/&lamba;Em (pH 9) = 495/519 nm</li>
<li>Yellow orange solid soluble in water (pH&gt;6), DMF or DMSO</li>
<li>Store at 4°C and protect from light</li>
<li>C₂₁H₁₂O₇</li>
<li>MW: 376.3</li>
<li>[3301-79-9]</li>
</ul>

Description & Target

6-Carboxy-X-rhodamine is a single isomer, long wavelength rhodamine dye. <ul> <li>&lamba;_Ex/&lamba;_Em(MeOH) = 568/595 nm</li> <li>Dark red solid soluble in DMSO, DMF or MeOH</li> <li>Store at -20°C and protect from light</li> <li>C₃₃H₃₀N₂O₅</li> <li>MW: 534.6</li> </ul>

Description & Target

6-ROX, SE is the amine-reactive form of 6-ROX single isomer dissolved in anhydrous DMSO, suitable for labeling oligonucleotides. <ul> <li>&lamba;_Ex/&lamba; _Em (MeOH) = 576/601 nm</li> <li>Dark red solution</li> <li>Store at -20°C and protect from moisture and light</li> <li>C₃₇H₃₃N₃O₇</li> <li>MW: 631.7</li> <li>[216699-36-4]</li> </ul>

Description & Target

6-SFX (full name: 6-(Fluorescein-6-carboxamido)hexanoic acid, succinimidyl ester, single isomer) has a 6-atom aminohexanoyl spacer group that separates the fluorescein dye and the amine-reactive group. <ul> <li>&lamba;_Ex/&lamba;_Em (pH 9) = 494/521 nm</li> <li>Yellow solid soluble in DMF, DMSO or pH&gt;6.5 buffers</li> <li>Store at -20°C and protect from light</li> <li>C₃₁H₂₆N₂O₁₀</li> <li>MW:586.55</li> </ul>

Description & Target

6-TAMRA SE (full name: 6-Carboxytetramethylrhodamine succinimidyl ester) is the amine-reactive form of 6-TAMRA single isomer. Supplied at 90 mg/mL (0.17 M) in anhydrous DMSO. The solution is suitable for labeling oligonucleotides. <ul> <li>&lamba;_Ex/&lamba;_Em (MeOH) = 540/565 nm</li> <li>Dark red solution</li> <li>Store desiccated at -20°C and protect from light</li> <li>C₂₉H₂₅N₃O₇</li> <li>MW: 527.5</li> </ul>

Description & Target

6X DNA Loading Buffer with Blue Tracking Dyes contains density agents and two blue tracking dyes that run around 1.5 kb and 200 bp in a 1% agarose gel. Mix 1 uL of 6X Loading Buffer for every 5 uL DNA sample before gel loading. 6X DNA Loading Buffer does not contain fluorescent DNA dye. 4 x 1.5 mL.

Description & Target

GelRed&reg; is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed&reg; Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed&reg; dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting.

The 6X GelRed&reg; Prestain Loading Buffers are available with blue tracking dyes or with orange tracking dye. Prestain buffer with blue tracking dyes (cat. no. 41009) contains two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel. Prestain buffer with orange tracking dye (cat. no. 41010) contains an orange electrophoresis tracking dye that runs at approximately 50 bp in a 1% agarose gel.

GelRed&reg; and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed&reg; without changing your existing imaging system. In addition, GelRed&reg; is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed&reg; is compatible with downstream applications such as sequencing and cloning. GelRed&reg; is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.