Description & Target

AM4-66 is a fixable red fluorescent nerve terminal probe. The dye is spectrally identical to SynaptoRed™ C2 (also called FM®4-64), but it has an additional amine group at the hydrophilic end of the molecule, making the dye fixable with formaldehyde or gluteraldehyde. The long emission wavelength makes the dye suitable to be used with GFP in two-color imaging.
<div>FM is a registered trademark of Thermo Fisher Scientific.</div>

Description & Target

AMCA-X SE is an amine-reactive, UV-excitable, blue fluorescent dye. <ul> <li>&lamba;_Ex/&lamba; _Em(MeOH) = 353/442 nm</li> <li>White solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C₂₂H₂₅N₃O₇</li> <li>MW: 443.45</li> </ul>

Description & Target

ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH₄) or sodium cyanoborohydride (NaB(CN)H₃) to form a stable linkage. This labeling technique has been widely used for the labeling and subsequent sequencing of oligosaccharides and glycoproteins. The negative charges of the dye facilitate the electrophoretic separation of the degradation products of carbohydrate polymers.

ANTS has also been used together with the fluorescent quencher DPX for the studies of membrane fusion or permeability. The mixture of ANTS-DPX is minimally fluorescent initially, but becomes increasingly more fluorescent upon dilution (i.e., membrane fusion or leakage).
<ul>
<li>&lamba;_Ex/&lamba;_Em = 353/520 nm</li>
<li>&epsilon;= 65,000</li>
<li>Light yellow solid soluble in water and DMSO</li>
<li>Store at 4°C and protect from light, especially in solution</li>
<li>C₁₀H₇NNa₂O₉S₃</li>
<li>MW: 427</li>
<li>[5398-34-5]</li>

Description & Target

The amine reactive succinimidyl ester of green fluorescent AO (acridine orange) can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugates then are able to complex with nucleic acids, resulting in green fluorescence nucleic acid conjugate adduct, making them potentially useful for studies of nucleic acid binding to various biomolecules, such as DNA-binding proteins.  It is also possible that conjugates of other biomolecules might be capable of monitoring their transport into the nucleus.  AO dye conjugates of solid or semisolid matrices, such as microspheres, magnetic particles or various resins, might be useful for the detection or affinity isolation of nucleic acids.

Description & Target

APTS is a very useful green fluorescent dye for labeling glycoproteins or sugar molecules in general. The labeling occurs via reductive amination which involves the formation of a Schiff base between the amine of APTS and the aldehyde or ketone of the sugar, followed by the reduction of the Schiff base linkage to a stable carbon-nitrogen bond. The multi-anionic nature of the dye makes it ideal for the studies of carbohydrate molecules by high resolution capillary electrophoresis. <ul> <li>&lamba; _Ex/&lamba; _Em: 424/505 nm (before conjugation)</li> <li>Yellow solid soluble in water</li> <li>Store at 4°C and protect from light.</li> <li>C₁₆H₈Na₃O₉S₃</li> <li>MW: 523.39</li> <li>[196504-57-1]</li> </ul>

Description & Target

ARP (N-(Aminooxyacetyl)-N'-(D-biotinoyl)hydrazine, trifluoroacetic acid salt) reacts with the exposed aldehyde group formed at abasic sites in damaged DNA, allowing the DNA to be labeled with biotin groups. The labeled DNA can then be quantitated with fluorescent or enzyme-conjugated streptavidin complexes. ARP can freely cross the cell membranes, thus allowing detection of abasic sites in living cells. ARP is suitable for use in microplate assays.
<ul>
<li>White solid soluble in DMSO</li>
<li>Store at 4°C</li>
<li>C₁₄H₂₂F₃N₅O₆S</li>
<li>MW: 445.41</li>
</ul>

Description & Target

ATP-Glo&trade; Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay procedure involves a single addition of ATP-Glo&trade; Detection Cocktail directly to cells cultured in a serum-supplemented medium. It is not necessary to remove cell culture medium or wash cells before adding the reagent.

Because ATP is an indicator of metabolically active cells, the number of viable cells can be assessed based on the amount of ATP available. This ATP detection kit takes advantage of firefly luciferase's use of ATP to oxidize D-Luciferin and the resulting production of light in order to assess the amount of ATP available. The ATP-Glo&trade; kit can be used to detect as little as a single cell or 0.01 picomole of ATP. The signal produced is linear within 6 orders of magnitude. By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from the determination of viable cell numbers to cell proliferation to cell cytotoxicity.

<strong>Please note: </strong>ATP-Glo&trade; is a flash-type luminescence assay. The luminescence signal generated is stable for up to 1 minute. This assay is designed for individual sample detection by using a luminometer in a single sample format or a luminometer with an injector in 96-well plate format.

<strong>Kit Components: </strong>
<ul>
<li>D-Luciferin</li>
<li>Firefly luciferase</li>
<li>ATP-Glo&trade; assay buffer</li>
<li>2 mM ATP standard</li>
</ul>
<p class="style5 style11"><strong>Features:</strong></p>

<ul>
<li><b>High Sensitivity and Extended Linearity: </b>Detect from a single cell to several million cells with excellent linearity</li>
<li><b>Simple:</b> A single-step homogenous assay</li>
<li><b>Versatile:</b> Detect ATP amount or quantify number of live cells</li>
</ul>

Description & Target

ATP-Glo&trade; Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay procedure involves a single addition of ATP-Glo&trade; Detection Cocktail directly to cells cultured in a serum-supplemented medium. It is not necessary to remove cell culture medium or wash cells before adding the reagent.

Because ATP is an indicator of metabolically active cells, the number of viable cells can be assessed based on the amount of ATP available. This ATP detection kit takes advantage of firefly luciferase's use of ATP to oxidize D-Luciferin and the resulting production of light in order to assess the amount of ATP available. The ATP-Glo&trade; kit can be used to detect as little as a single cell or 0.01 picomole of ATP. The signal produced is linear within 6 orders of magnitude. By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from the determination of viable cell numbers to cell proliferation to cell cytotoxicity.

<strong>Please note: </strong>ATP-Glo&trade; is a flash-type luminescence assay. The luminescence signal generated is stable for up to 1 minute. This assay is designed for individual sample detection by using a luminometer in a single sample format or a luminometer with an injector in 96-well plate format.

<strong>Kit Components: </strong>
<ul>
<li>D-Luciferin</li>
<li>Firefly luciferase</li>
<li>ATP-Glo&trade; assay buffer</li>
<li>2 mM ATP standard</li>
</ul>
<p class="style5 style11"><strong>Features:</strong></p>

<ul>
<li><b>High Sensitivity and Extended Linearity: </b>Detect from a single cell to several million cells with excellent linearity</li>
<li><b>Simple:</b> A single-step homogenous assay</li>
<li><b>Versatile:</b> Detect ATP amount or quantify number of live cells</li>
</ul>

Description & Target

ATP-Glo&trade; Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay procedure involves a single addition of ATP-Glo&trade; Detection Cocktail directly to cells cultured in a serum-supplemented medium. It is not necessary to remove cell culture medium or wash cells before adding the reagent.

Because ATP is an indicator of metabolically active cells, the number of viable cells can be assessed based on the amount of ATP available. This ATP detection kit takes advantage of firefly luciferase's use of ATP to oxidize D-Luciferin and the resulting production of light in order to assess the amount of ATP available. The ATP-Glo&trade; kit can be used to detect as little as a single cell or 0.01 picomole of ATP. The signal produced is linear within 6 orders of magnitude. By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from the determination of viable cell numbers to cell proliferation to cell cytotoxicity.

<strong>Please note: </strong>ATP-Glo&trade; is a flash-type luminescence assay. The luminescence signal generated is stable for up to 1 minute. This assay is designed for individual sample detection by using a luminometer in a single sample format or a luminometer with an injector in 96-well plate format.

<strong>Kit Components: </strong>
<ul>
<li>D-Luciferin</li>
<li>Firefly luciferase</li>
<li>ATP-Glo&trade; assay buffer</li>
<li>2 mM ATP standard</li>
</ul>
<p class="style5 style11"><strong>Features:</strong></p>

<ul>
<li><b>High Sensitivity and Extended Linearity: </b>Detect from a single cell to several million cells with excellent linearity</li>
<li><b>Simple:</b> A single-step homogenous assay</li>
<li><b>Versatile:</b> Detect ATP amount or quantify number of live cells</li>
</ul>

Description & Target

Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.<ul> <li>Soluble in DMSO</li> <li>Store at 4&deg;C</li> <li>C₂₀H₃₀N₄O₁₁</li> <li>MW: 502.5</li></ul>

Description & Target

Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.<ul> <li>Soluble in DMSO</li> <li>Store at 4&deg;C</li> <li>C₂₀H₃₀N₄O₁₁</li> <li>MW: 502.5</li></ul>

Description & Target

(Ac-DEVD)2-Rhodamine 110 contains two DEVD tetrapeptides and is hydrolyzed by caspase-3/7 in two successive steps to release the green fluorescent dye rhodamine 110 (R110) (Ex/Em 496/520 nm). Unit size: 1 mg.

Description & Target

Z-IETD-R110 is a substrate for caspase-8, which is involved at the early stage of apoptosis, acting as an initiator of the caspase activation cascade.<ul><li>Full name: Rhodamine 110, bis-(N-acetyl-L-isoleucyl-L-glutamyl-L-threonyl-L-aspartic acid amide</li><li>&lambda; _Ex/&lambda; _Em of end product (R110) = 496/520 nm</li><li>Off-white to pink solid soluble in DMSO</li><li>C₆₈H₈₂N₁₀O₂₄</li><li>MW: 1422</li></ul>

Description & Target

The AccuBlue Broad Range RNA Quantitation Kit is designed for RNA Quantitation on a fluorescent plate reader. The AccuBlue RNA Quantitation Kit comes with Dye, Buffer, RNA Standard and dilution buffer. It provides accurate RNA-specific quantitation of purified samples.

Description & Target

The AccuBlue Broad Range RNA Quantitation Kit is designed for RNA Quantitation on a fluorescent plate reader. The AccuBlue RNA Quantitation Kit comes with Dye, Buffer, RNA Standard and dilution buffer. It provides accurate RNA-specific quantitation of purified samples.