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Biotium
AccuBlue® Broad Range dsDNA Quantitation Kit

SKU

31007

Size

1KIT

$255

Description & Target

The AccuBlue Broad Range dsDNA Quantitation Kit provides reagents for 1500 microplate assays. This assay is intended for the quantification of dsDNA in the range of 2-2000 ng/uL.

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AccuBlue® Broad Range dsDNA Quantitation Kit with DNA Standard, trial size (200

SKU

31007-T

Size

1KIT

$58

Description & Target

The AccuBlue® Broad Range dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants.  The kit provides a linear detection range from 2 - 2000 ng and is compatible with fluorescence microplate readers that can detect blue fluorescence. AccuBlue® Broad Range dsDNA Quantitation Solution also is available separately for customers who wish to use their own DNA standards.

For broad range DNA quantitation using the Qubit® fluorometer from Thermo Fisher, we recommend using our AccuGreen Broad Range dsDNA Quantitation Kit.

For high-sensitivity DNA quantitation using a fluorescent plate reader, we recommend using our AccuClear® or AccuBlue® NextGen dsDNA Quantitation Kits, which provide unprecedented sensitivity, accuracy and wide linear range. See the table below for details on our full line of dsDNA quantitation kits.

<strong>Features</strong>
<ul>
<li>Broad linear detection range, from 2-2000 ng</li>
<li>Blue fluorescence emission (Ex/Em: 350/460 nm)</li>
</ul>

<strong>Kit Components </strong>

<strong>200 Assays (Trial Size)</strong>
<ul>
<li>AccuBlue&reg; Broad Range Dye, 100X</li>
<li>AccuBlue&reg; Broad Range Buffer, 1X</li>
<li>AccuBlue&reg; Broad Range Enhancer, 100X</li>
<li>AccuBlue&reg; dsDNA Standard, 200 ng/uL</li>
</ul>

<strong>1000 Assays</strong>
<ul>
<li>AccuBlue&reg; Broad Range Dye, 100X</li>
<li>AccuBlue&reg; Broad Range Buffer, 1X</li>
<li>AccuBlue&reg; Broad Range Enhancer, 100X</li>
<li>AccuBlue&reg; dsDNA Standards, set of 9</li>
</ul>

AccuBlue and AccuClear are registered trademarks of 9689c5aa-6556-4b3c-b5d1-1e693715ba07, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.

Molecular Weight

Molecular Formula

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AccuBlue® Broad Range dsDNA Quantitation Solution

SKU

31009

Size

1KIT

$171

Description & Target

The AccuBlue Broad Range dsDNA Quantitation Kit provides reagents for 1500 microplate assays. This assay is intended for the quantification of dsDNA in the range of 2-2000 ng/uL.

Molecular Weight

Molecular Formula

Species

CAS Number

Tag / Host

Expression

AccuBlue® Broad Range dsDNA Quantitation Solution, trial size

SKU

31009-T

Size

1KIT

$45

Description & Target

The AccuBlue&reg; Broad Range dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants.  The solution provides a linear detection range from 2 - 2000 ng and is compatible with fluorescence microplate readers that can detect blue fluorescence. This item is a standalone fluorescent DNA quantitation solution, and does not include DNA standards. It is intended for users who wish to use their own DNA standards. We also sell the complete AccuBlue&reg; Broad Range Quantitation Kit that includes a set of standards.

For broad range DNA quantitation using the Qubit&reg; fluorometer from Thermo Fisher, we recommend using our AccuGreen Broad Range dsDNA Quantitation Kit. For high-sensitivity DNA quantitation using a fluorescent plate reader, we recommend using our AccuClear&reg; or AccuBlue&reg; NextGen dsDNA Quantitation Kits, which provide unprecedented sensitivity, accuracy and wide linear range.

<strong>Features</strong>

<ul>
<li>Broad linear detection range, from 2-2000 ng</li>
<li>Blue fluorescence emission (Ex/Em: 350/460 nm)</li>

</ul>

<strong>Components</strong>

<ul>
<li>AccuBlue&reg; Broad Range Dye, 100X</li>
<li>AccuBlue&reg; Broad Range Buffer, 1X</li>
<li>AccuBlue&reg; Broad Range Enhancer, 100X</li>
</ul>

AccuBlue and AccuClear are registered trademarks of 9689c5aa-6556-4b3c-b5d1-1e693715ba07, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.

Molecular Weight

Molecular Formula

Species

CAS Number

Tag / Host

Expression

AccuBlue® Broad Range dsDNA Quantitation Standards, set of nine, 0.5 mL each

SKU

31007C

Size

1KIT

$84

Description & Target

Stand-alone set of the nine double-stranded DNA standards provided with the AccuBlue&reg; Broad Range dsDNA Quantitation Kit. Calf thymus dsDNA in 10 mM Tris pH 7.5, 1 mM EDTA, 2 mM sodium azide at 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL, 0.5 mL each.

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AccuBlue® High Sensitivity dsDNA Quantitation Kit

SKU

31006

Size

1KIT

$255

Description & Target

AccuBlue&reg; High Sensitivity dsDNA Quantitation Assay is a safer fluorescent dsDNA quantitation assay for microplate reader. The assay uses non-toxic, non-mutagenic EvaGreen&reg; dye. Featuring our unique AccuBlue&reg; High Sensitivity Enhancer, the assay provides highly sensitive and selective detection of purified dsDNA samples with excellent linearity. The kits include calf thymus dsDNA standards, and provide sufficient reagents to perform 200 assays (trial size) or 1000 assays (regular size) based on a 96-well microplate format. AccuBlue&reg; High Sensitivity dsDNA Quantitation Solution also is available without standards for customers who wish to use their own DNA standards.

The assay is compatible with microplate readers, spectrofluorometers and hand-held minifluorometers. Note that while the assay is spectrally compatible with Qubit&reg; fluorometers from Thermo Fisher, it may not be compatible with the high sensitivity dsDNA program on all Qubit&reg; models. For the Qubit&reg; we recommend our AccuGreen&trade; High Sensitivity Kit, which was specially formulated for that instrument.

<strong>Features</strong>
<ul>
<li>Linear detection range from 0.2 - 100 ng</li>
<li>Non-toxic, non-mutagenic EvaGreen&reg; dye - safer than PicoGreen&reg; or Quant-iT&reg; kits</li>
<li>AccuBlue&reg; High Sensitivity Enhancer provides high sensitivity and excellent linearity</li>
</ul>

<strong>Kit Components </strong>

<strong>200 Assays (Trial Size)</strong>
<ul>
<li>AccuBlue&reg; High Sensitivity dsDNA Quantitation Solution, 1X</li>
<li>AccuBlue&reg; High Sensitivity Enhancer, 100X</li>
<li>AccuBlue&reg; dsDNA Standard, 10 ng/uL</li>
</ul>

<strong>1000 Assays</strong>
<ul>
<li>AccuBlue&reg; High Sensitivity dsDNA Quantitation Solution, 1X</li>
<li>AccuBlue&reg; High Sensitivity Enhancer, 100X</li>
<li>AccuBlue&reg; dsDNA Standards, set of 8</li>
</ul>

AccuBlue and AccuClear are registered trademarks of 9689c5aa-6556-4b3c-b5d1-1e693715ba07, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.

Molecular Weight

Molecular Formula

Species

CAS Number

Tag / Host

Expression

AccuBlue® High Sensitivity dsDNA Quantitation Kit with DNA Standard, trial size

SKU

31006-T

Size

1KIT

$58

Description & Target

AccuBlue&reg; High Sensitivity dsDNA Quantitation Assay is a safer fluorescent dsDNA quantitation assay for microplate reader. The assay uses non-toxic, non-mutagenic EvaGreen&reg; dye. Featuring our unique AccuBlue&reg; High Sensitivity Enhancer, the assay provides highly sensitive and selective detection of purified dsDNA samples with excellent linearity. The kits include calf thymus dsDNA standards, and provide sufficient reagents to perform 200 assays (trial size) or 1000 assays (regular size) based on a 96-well microplate format. AccuBlue&reg; High Sensitivity dsDNA Quantitation Solution also is available without standards for customers who wish to use their own DNA standards.

The assay is compatible with microplate readers, spectrofluorometers and hand-held minifluorometers. Note that while the assay is spectrally compatible with Qubit&reg; fluorometers from Thermo Fisher, it may not be compatible with the high sensitivity dsDNA program on all Qubit&reg; models. For the Qubit&reg; we recommend our AccuGreen&trade; High Sensitivity Kit, which was specially formulated for that instrument.

<strong>Features</strong>
<ul>
<li>Linear detection range from 0.2 - 100 ng</li>
<li>Non-toxic, non-mutagenic EvaGreen&reg; dye - safer than PicoGreen&reg; or Quant-iT&reg; kits</li>
<li>AccuBlue&reg; High Sensitivity Enhancer provides high sensitivity and excellent linearity</li>
</ul>

<strong>Kit Components </strong>

<strong>200 Assays (Trial Size)</strong>
<ul>
<li>AccuBlue&reg; High Sensitivity dsDNA Quantitation Solution, 1X</li>
<li>AccuBlue&reg; High Sensitivity Enhancer, 100X</li>
<li>AccuBlue&reg; dsDNA Standard, 10 ng/uL</li>
</ul>

<strong>1000 Assays</strong>
<ul>
<li>AccuBlue&reg; High Sensitivity dsDNA Quantitation Solution, 1X</li>
<li>AccuBlue&reg; High Sensitivity Enhancer, 100X</li>
<li>AccuBlue&reg; dsDNA Standards, set of 8</li>
</ul>

AccuBlue and AccuClear are registered trademarks of 9689c5aa-6556-4b3c-b5d1-1e693715ba07, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.

Molecular Weight

Molecular Formula

Species

CAS Number

Tag / Host

Expression

AccuBlue® High Sensitivity dsDNA Quantitation Solution

SKU

31008

Size

1KIT

$171

Description & Target

The AccuBlue&reg;  High Sensitivity dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The Solution provides sufficient reagents to perform 1000 assays based on a 96-well microplate format. This item is a standalone fluorescent DNA quantitation solution, and is intended for customers who wish to use their own DNA standards. We also sell the complete AccuBlue&reg; High Sensitivity Quantitation Kit that includes a set of calf thymus dsDNA standards.

The assay is compatible with microplate readers, spectrofluorometers and hand-held minifluorometers. Note that while the assay is spectrally compatible with Qubit&reg; fluorometers from Thermo Fisher, it may not be compatible with the high sensitivity dsDNA program on all Qubit&reg; models. For the Qubit&reg; fluorometer we recommend our AccuGreen&trade; High Sensitivity Kit, which was specially formulated for that instrument.

<strong>Features</strong>
<ul>
<li>linear detection range from 0.2 - 100 ng</li>
<li>Safer than PicoGreen&reg; or Quant-iT&reg; dsDNA detection kits: the high sensitivity reagent is impermeable to cell membranes</li>
</ul>
<strong>Components</strong>
<ul>
<li>AccuBlue&reg; High Sensitivity dsDNA Quantitation Solution, 1X</li>
<li>AccuBlue&reg; High Sensitivity Enhancer, 100X</li>

AccuBlue and AccuClear are registered trademarks of 9689c5aa-6556-4b3c-b5d1-1e693715ba07, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.

Molecular Weight

Molecular Formula

Species

CAS Number

Tag / Host

Expression

AccuBlue® High Sensitivity dsDNA Quantitation Solution, trial size

SKU

31008-T

Size

1KIT

$45

Description & Target

The AccuBlue&reg;  High Sensitivity dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The Solution provides sufficient reagents to perform 1000 assays based on a 96-well microplate format. This item is a standalone fluorescent DNA quantitation solution, and is intended for customers who wish to use their own DNA standards. We also sell the complete AccuBlue&reg; High Sensitivity Quantitation Kit that includes a set of calf thymus dsDNA standards.

The assay is compatible with microplate readers, spectrofluorometers and hand-held minifluorometers. Note that while the assay is spectrally compatible with Qubit&reg; fluorometers from Thermo Fisher, it may not be compatible with the high sensitivity dsDNA program on all Qubit&reg; models. For the Qubit&reg; fluorometer we recommend our AccuGreen&trade; High Sensitivity Kit, which was specially formulated for that instrument.

<strong>Features</strong>
<ul>
<li>linear detection range from 0.2 - 100 ng</li>
<li>Safer than PicoGreen&reg; or Quant-iT&reg; dsDNA detection kits: the high sensitivity reagent is impermeable to cell membranes</li>
</ul>
<strong>Components</strong>
<ul>
<li>AccuBlue&reg; High Sensitivity dsDNA Quantitation Solution, 1X</li>
<li>AccuBlue&reg; High Sensitivity Enhancer, 100X</li>

AccuBlue and AccuClear are registered trademarks of 9689c5aa-6556-4b3c-b5d1-1e693715ba07, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.

Molecular Weight

Molecular Formula

Species

CAS Number

Tag / Host

Expression

AccuBlue® High Sensitivity dsDNA Standards, set of eight, 0.5 mL each

SKU

31006C

Size

1KIT

$84

Description & Target

Stand-alone set of the eight double-stranded DNA standards provided with the AccuBlue&reg; High Sensitivity dsDNA Quantitation Kit. Calf thymus dsDNA in 10 mM Tris pH 7.5, 1 mM EDTA, 2 mM sodium azide at 0, 0.5, 1, 2, 4, 6, 8 and 10 ng/uL, 0.5 mL each.

Molecular Weight

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Species

CAS Number

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AccuBlue® NextGen dsDNA Quantitation Kit (1000 assays)

SKU

31060

Size

1kit

$397

Description & Target

AccuBlue NextGen dsDNA Quantitation is the most sensitive DNA quantitation assay on the market, with a detection limit down to 1 pg depending on the instrument. Available as a stand-alone quantitation solution, or as a kit with a calf thymus dsDNA standard (catalog no. 31060).

Molecular Weight

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Species

CAS Number

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AccuBlue® NextGen dsDNA Quantitation Kit, Trial Size (200 assays)

SKU

31060-T

Size

1kit

$91

Description & Target

AccuBlue NextGen dsDNA Quantitation is the most sensitive DNA quantitation assay on the market, with a detection limit down to 1 pg depending on the instrument. Available as a stand-alone quantitation solution, or as a kit with a calf thymus dsDNA standard (catalog no. 31060).

Molecular Weight

Molecular Formula

Species

CAS Number

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Expression

AccuBlue® NextGen dsDNA Quantitation Solution (1000 assays)

SKU

31061

Size

1kit

$261

Description & Target

AccuBlue NextGen dsDNA Quantitation is the most sensitive DNA quantitation assay on the market, with a detection limit down to 1 pg depending on the instrument. Available as a stand-alone quantitation solution, or as a kit with a calf thymus dsDNA standard (catalog no. 31060).

Molecular Weight

Molecular Formula

Species

CAS Number

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Expression

AccuBlue® NextGen dsDNA Quantitation Solution, Trial Size (200 assays)

SKU

31061-T

Size

1kit

$63

Description & Target

AccuBlue NextGen dsDNA Quantitation is the most sensitive DNA quantitation assay on the market, with a detection limit down to 1 pg depending on the instrument. Available as a stand-alone quantitation solution, or as a kit with a calf thymus dsDNA standard (catalog no. 31060).

Molecular Weight

Molecular Formula

Species

CAS Number

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Expression

AccuClear® Ultra High Sensitivity dsDNA Quantitation Assay kit with 1 DNA Standa

SKU

31029

Size

1kit

$451

Description & Target

AccuClear&reg; Ultra High Sensitivity dsDNA Quantitation Kit utilizes a novel, next-generation DNA binding dye for highly sensitive and accurate quantitation of purified dsDNA samples across a broad range of DNA concentrations. The assay is linear between 0.03 ng and 250 ng of dsDNA per assay in microplate format, making it the most versatile DNA quantitation assay. Unlike absorbance-based measurements, AccuClear&reg; dye is highly selective for double-stranded DNA over single stranded DNA or RNA. We also sell a stand-alone AccuClear&reg; Quantitation Solution without DNA standards, for users who wish to use their own standards.

The AccuClear&reg; dsDNA quantitation assay is designed for use with fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuClear&reg; dye make it especially well-suited for use with instruments with blue LED excitation sources. For DNA quantitation using the Qubit&reg; fluorometer we recommend our AccuGreen&trade; High Sensitivity dsDNA Quantitation Kit and AccuGreen&trade; Broad Range dsDNA Quantitation Kit, which were formulated specifically for that instrument.

<b>Features</b>
<ul>
<li>Linear range: 0.03-250 ng dsDNA</li>
<li>Unrivaled sensitivity and dynamic range</li>
</ul>

<b>Kit Components:</b>

<strong>200 Assays (Trial Size)</strong>
<ul>
<li>AccuClear&reg; Dye, 100X</li>
<li>AccuClear&reg; Buffer, 1X</li>
<li>25 ng/uL dsDNA Standard</li>
</ul>

<p style="text-align: left;"><strong>1000 Assays</strong></p>
<ul>
<li>AccuClear&reg; Dye, 100X</li>
<li>AccuClear&reg; Buffer, 1X</li>
<li>Set of 7 dsDNA Standards</li>
</ul>

<p style="text-align: left;"><strong>2000 Assays</strong></p>
<ul>
<li>AccuClear&reg; Dye, 100X</li>
<li>AccuClear&reg; Buffer, 20X</li>
<li>25 ng/uL dsDNA Standard</li>

<div>AccuBlue and AccuClear are registered trademarks of 9689c5aa-6556-4b3c-b5d1-1e693715ba07, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.</div>

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Molecular Formula

Species

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