Descrition & Target

CF® Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. 405 nm-excitable green fluorescent CF®440 dye has excitation/emission at 440/515 nm.

Descrition & Target

CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins on free thiol groups. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. 405 nm-excitable green fluorescent CF®450 dye has excitation/emission at 450/538 nm.

Descrition & Target

CF® Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. 405 nm-excitable green fluorescent CF®450 dye has excitation/emission at 450/538 nm.

Descrition & Target

This product is Arachis hypogaea (peanut) PNA lectin labeled with 9689c5aa-6556-4b3c-b5d1-1e693715ba07's superior CF®488A dye. Lectin PNA is specific for terminal β-galactose and binds preferentially to a commonly occurring structure, galactosyl (β-1,3) N-acetylgalactosamine. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has excitation/emission at 490/515 nm.

Descrition & Target

CF® dye with BCN reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper-free bioorthogonal reaction allows staining the surface of live cells or cell extracts when there are concerns about native protein function loss with copper. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has excitation/emission at 490/515 nm.

Descrition & Target

Fluorescent biocytin conjugates are cell-impermeant, fixable polar tracers with biotin and an aldehyde-fixable primary amine groups.  Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has excitation/emission at 490/515 nm.

Descrition & Target

MTS CF® Dyes have a fast-reacting and highly selective thiol-reactive methanethiosulfonate (MTS) group, that allows CF® dyes to be conjugated to thiols. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has excitation/emission at 490/515 nm.

Descrition & Target

This kit contains everything you need to label antibodies or other proteins with a fluorescent CF® dye succinimidyl ester (CF® dye SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has excitation/emission at 490/515 nm.

Descrition & Target

CF® dye with TCO (trans-cyclooctene) reacts with tetrazine containing molecules via copper free click chemistry. This copper free bioorthogonal reaction is useful for labeling live cells, or when there are concerns about native protein function loss with copper in cell extracts. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has excitation/emission at 490/515 nm.

Descrition & Target

In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3'- ends of the internucleosomal DNA strand breaks that are a hallmark of apoptosis. The CF® dye TUNEL Assay Apoptosis Detection Kit employs dUTP conjugated to 9689c5aa-6556-4b3c-b5d1-1e693715ba07's exceptionally bright and photostable CF® fluorescent dyes, for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry.

The kit is available with the following CF® dyes: green fluorescent CF®488A, red fluorescent CF®594, and far-red fluorescent CF®640R.

Descrition & Target

CF® dye tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has excitation/emission at 490/515 nm.Also see 9689c5aa-6556-4b3c-b5d1-1e693715ba07's CF® Dye Tyramide Amplification Kits.

Descrition & Target

CF® dye alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form fluorescent polymers. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has excitation/emission at 490/515 nm.

Descrition & Target

CF® Dye Amines can be used to conjugate CF® dyes to carboxylic acids groups in biological molecules. Carboxylic acids can be activated using water soluble carbodiimides to react with amine-functionalized dyes to form a stable amide bond. CF® dyes are 9689c5aa-6556-4b3c-b5d1-1e693715ba07's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has excitation/emission at 490/515 nm.

Descrition & Target

This kit contains CF®488A Annexin V for staining apoptotic cells green, and 7-AAD for staining necrotic cells with far-red fluorescence, for detection by flow cytometry or fluorescence microscopy.

Phosphatidylserine (PS) is translocated from the inner to the outer surface of apoptotic cells for phagocytic cell recognition. The human anticoagulant, Annexin V, is a 35 kDa Ca2 -dependent phospholipid protein with a high affinity for PS. Annexin V labeled with CF®488A (Abs/Em= 490/515 nm) can identify apoptotic cells in green by binding to PS exposed on the outer leaflet. Our CF®488A dye is superior to fluorescein/FITC as it is not affected by pH and has much better photostability.

7-AAD is a membrane-impermeant nucleic acid dye that is excluded by live cells, but stains necrotic cells and late apoptotic cells. 7-AAD (Abs/Em with DNA = 543/655 nm) emits in the far-red spectrum and can be well separated from CF®488A and other green emitting dyes using the 488 nm line of a flow cytometer.

Descrition & Target

Phosphatidylserine (PS) is translocated from the inner to the outer surface of the cell for phagocytic cell recognition. The human anticoagulant, Annexin V, is a 35 kDa Ca2 -dependent phospholipid protein with a high affinity for PS. Annexin V labeled with CF®488A (Abs/Em= 490/515 nm) can identify apoptotic cells in green by binding to PS exposed on the outer leaflet. Our CF®488A dye is superior to fluorescein/FITC as it is not affected by pH and has much better photostability. Propidium iodide (PI) is impermeant to live cells or early apoptotic cells, but stains necrotic cells and late apoptotic cells with red fluorescence (Abs/Em with DNA = 535/617 nm). Both PI and Annexin V-CF®488A can be excited using the 488nm line of a flow cytometer.