Diagnostic & Veterinary
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Coelenterazine (ALSO KNOWN AS NATIVE COE
Descrition & Target
Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. The aequorin complex has been used as an excellent calcium indicator that can measure a broad concentration range of calcium from <em>~</em>0.1 uM to >100 uM. Renilla luciferase has been widely used as a reporter protein. Renilla luciferase/coelenterazine has also been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Other uses of coelenterazine include chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues. Also see water soluble Aquaphile™ Coelenterazine.<ul> <li>Yellow solid soluble in MeOH or EtOH</li> <li>Store at -20°C and protect from light</li> <li>C<sub>26</sub>H<sub>21</sub>N<sub>3</sub>O<sub>3</sub></li> <li>MW: 423</li> <li>[55779-48-1]</li></ul>
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Expression
Coelenterazine (ALSO KNOWN AS NATIVE COE
Descrition & Target
Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. The aequorin complex has been used as an excellent calcium indicator that can measure a broad concentration range of calcium from <em>~</em>0.1 uM to >100 uM. Renilla luciferase has been widely used as a reporter protein. Renilla luciferase/coelenterazine has also been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Other uses of coelenterazine include chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues. Also see water soluble Aquaphile™ Coelenterazine.<ul> <li>Yellow solid soluble in MeOH or EtOH</li> <li>Store at -20°C and protect from light</li> <li>C<sub>26</sub>H<sub>21</sub>N<sub>3</sub>O<sub>3</sub></li> <li>MW: 423</li> <li>[55779-48-1]</li></ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
Coelenterazine (ALSO KNOWN AS NATIVE COE
Descrition & Target
Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. The aequorin complex has been used as an excellent calcium indicator that can measure a broad concentration range of calcium from <em>~</em>0.1 uM to >100 uM. Renilla luciferase has been widely used as a reporter protein. Renilla luciferase/coelenterazine has also been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Other uses of coelenterazine include chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues. Also see water soluble Aquaphile™ Coelenterazine.<ul> <li>Yellow solid soluble in MeOH or EtOH</li> <li>Store at -20°C and protect from light</li> <li>C<sub>26</sub>H<sub>21</sub>N<sub>3</sub>O<sub>3</sub></li> <li>MW: 423</li> <li>[55779-48-1]</li></ul>
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Expression
Coelenterazine 400A (DEEPBLUEC, A TRADEM
Descrition & Target
Coelenterazine 400a, also called DeepBlueC™, is a coelenterazine derivative that serves as a substrate for <i>Renilla luciferase</i> (Rluc) and generates an emission peak centered around 400 nm. It is the preferred Rluc substrate for BRET studies because it has minimal interference with the emission of the GFP acceptor. <ul> <li>Yellow solid soluble in MeOH or EtOH</li> <li>Store at 20°C and protect from light</li> <li>C<sub>26</sub>H<sub>21</sub>N<sub>3</sub>O</li> <li>MW: 391.46</li></ul>DeepBlueC is a trademark of BioSignal Packard.
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Coelenterazine 400A (DEEPBLUEC, A TRADEM
Descrition & Target
Coelenterazine 400a, also called DeepBlueC™, is a coelenterazine derivative that serves as a substrate for <i>Renilla luciferase</i> (Rluc) and generates an emission peak centered around 400 nm. It is the preferred Rluc substrate for BRET studies because it has minimal interference with the emission of the GFP acceptor. <ul> <li>Yellow solid soluble in MeOH or EtOH</li> <li>Store at 20°C and protect from light</li> <li>C<sub>26</sub>H<sub>21</sub>N<sub>3</sub>O</li> <li>MW: 391.46</li></ul>DeepBlueC is a trademark of BioSignal Packard.
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Coelenterazine 400A (DEEPBLUEC, A TRADEM
Descrition & Target
Coelenterazine 400a, also called DeepBlueC™, is a coelenterazine derivative that serves as a substrate for <i>Renilla luciferase</i> (Rluc) and generates an emission peak centered around 400 nm. It is the preferred Rluc substrate for BRET studies because it has minimal interference with the emission of the GFP acceptor. <ul> <li>Yellow solid soluble in MeOH or EtOH</li> <li>Store at 20°C and protect from light</li> <li>C<sub>26</sub>H<sub>21</sub>N<sub>3</sub>O</li> <li>MW: 391.46</li></ul>DeepBlueC is a trademark of BioSignal Packard.
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Coelenterazine CP
Descrition & Target
Coelenterazine cp is a synthetic derivative of coelenterazine. Its aequorin complex generates luminescence intensity 15 times higher and has a faster response time to calcium compared to native coelenterazine.<ul><li>Yellow solid soluble in MeOH or EtOH</li><li>Store at -20°C and protect from light</li><li>C<sub>25</sub>H<sub>25</sub>N<sub>3</sub>O<sub>3</sub></li><li>MW: 415.48</li></ul>
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Coelenterazine CP
Descrition & Target
Coelenterazine cp is a synthetic derivative of coelenterazine. Its aequorin complex generates luminescence intensity 15 times higher and has a faster response time to calcium compared to native coelenterazine.<ul><li>Yellow solid soluble in MeOH or EtOH</li><li>Store at -20°C and protect from light</li><li>C<sub>25</sub>H<sub>25</sub>N<sub>3</sub>O<sub>3</sub></li><li>MW: 415.48</li></ul>
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Molecular Weight
Molecular Formula
Tag / Host
Expression
Coelenterazine CP
Descrition & Target
Coelenterazine cp is a synthetic derivative of coelenterazine. Its aequorin complex generates luminescence intensity 15 times higher and has a faster response time to calcium compared to native coelenterazine.<ul><li>Yellow solid soluble in MeOH or EtOH</li><li>Store at -20°C and protect from light</li><li>C<sub>25</sub>H<sub>25</sub>N<sub>3</sub>O<sub>3</sub></li><li>MW: 415.48</li></ul>
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Coelenterazine E
Descrition & Target
Coelenterazine e is a synthetic derivative of coelenterazine, and is a superior substrate for Renilla luciferase. Coelenterazine e generates nearly 40% more light than native coelenterazine does while the initial light intensity of the former is more than 7 times higher than that of the latter. The emission spectrum of coelenterazine e/Renilla luciferase has two peaks centered at 418 and 475 nm, respectively.Coelenterazine e is also a good substrate for apoaequorin and, compared with coelenterazine native, the e-aequorin compelx formed from coelenterazine e has a faster luminescence rise time and shows two emission peaks at 405 and 465 nm respectively, with the ratio of the peak heights dependent on calcium concentration in the range pCa 5-7. The dual emission makes coelenterazine e/apoaequorin possible to be used for calcium detection using the ratio of the two emission intensities.<ul> <li>Yellow solid soluble in MeOH or EtOH</li> <li>Store at -20°C and protect from light</li> <li>C<sub>28</sub>H<sub>23</sub>N<sub>3</sub>O<sub>3</sub></li> <li>MW: 449.50</li></ul>
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Coelenterazine E
Descrition & Target
Coelenterazine e is a synthetic derivative of coelenterazine, and is a superior substrate for Renilla luciferase. Coelenterazine e generates nearly 40% more light than native coelenterazine does while the initial light intensity of the former is more than 7 times higher than that of the latter. The emission spectrum of coelenterazine e/Renilla luciferase has two peaks centered at 418 and 475 nm, respectively.Coelenterazine e is also a good substrate for apoaequorin and, compared with coelenterazine native, the e-aequorin compelx formed from coelenterazine e has a faster luminescence rise time and shows two emission peaks at 405 and 465 nm respectively, with the ratio of the peak heights dependent on calcium concentration in the range pCa 5-7. The dual emission makes coelenterazine e/apoaequorin possible to be used for calcium detection using the ratio of the two emission intensities.<ul> <li>Yellow solid soluble in MeOH or EtOH</li> <li>Store at -20°C and protect from light</li> <li>C<sub>28</sub>H<sub>23</sub>N<sub>3</sub>O<sub>3</sub></li> <li>MW: 449.50</li></ul>
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