Diagnostic & Veterinary
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DAPI
Descrition & Target
DAPI (4',6-Diamidino-2-Phenylindole, dihydrochloride) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Also see DAPI dilactate, a more water soluble form of the dye.
<ul>
<li>Classic blue nuclear counterstain for fixed cells</li>
<li>Can be used at higher concentrations to stain live cells</li>
<li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em</sub> = 358/461 nm (with DNA)</li>
<li>Off-white solid soluble in H<sub>2</sub>O</li>
<li>Store at 4°C and protect from light, especially in solution</li>
<li>C<sub>16</sub>H<sub>17</sub>Cl<sub>2</sub>N<sub>5</sub></li>
<li>MW = 350.25</li>
<li>[28718-90-3]</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
DAPI, 10mg/mL in H2O
Descrition & Target
DAPI (4',6-Diamidino-2-Phenylindole) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Because of the potential toxicity of DAPI, we offer DAPI dilactate dissolved in H<sub>2</sub>O as a safer alternative to weighing out the powdery form. We also offer DAPI dilactate solid, which is more water soluble than the dihydrochloride form of the dye.
<ul>
<li>Classic blue nuclear counterstain for fixed cells</li>
<li>Can be used at higher concentrations to stain live cells</li>
<li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em </sub>(with DNA) = 358/461 nm</li>
<li>Yellow solution</li>
<li>Store at 4°C and protect from light</li>
<li>C<sub>22</sub>H<sub>27</sub>N<sub>5</sub>O<sub>6</sub></li>
<li>MW: 457.49</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
DAPI, dilactate (4’,6-diamidino-2-phenylindole, dilactate)
Descrition & Target
DAPI is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. DAPI dilactate is essentially the same as DAPI dihydrochloride, but with a different counterion. The dilactate salt form of the dye is more water soluble than the dihydrochloride salt, and therefore DAPI dilactate is a better choice for preparing stock solutions in water.
<ul>
<li>Classic blue nuclear counterstain for fixed cells</li>
<li>Can be used at higher concentrations to stain live cells</li>
<li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em</sub>(DNA) = 358/461 nm</li>
<li>Yellow solid soluble in water</li>
<li>Store at 4°C and protect from light, especially in solution</li>
<li>C<sub>22</sub>H<sub>27</sub>N<sub>5</sub>O<sub>6</sub></li>
<li>MW: 457.49</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression
DASPEI
Descrition & Target
DASPEI (2-(4-(Dimethylamino)styryl)-N-ethylpyridinium iodide) is used to stain mitochondria in live cells.
<ul>
<li>&lamba;<sub>Ex</sub>/&lamba; <sub>Em</sub> = 461/589 nm (MeOH)</li>
<li>Store at 4°C and protect from light, especially in solution</li>
<li>C<sub>17</sub>H<sub>21</sub>IN<sub>2</sub></li>
<li>MW: 380</li>
<li>[3785-01-1]</li>
</ul>
CAS Number
Molecular Weight
Molecular Formula
Tag / Host
Expression