Descrition & Target

DiD (DiIC18(5); 1,1'-dioctadecyl-3,3,3',3'- tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt) is a lipophilic carbocyanine dye similar to DiI, but with longer absorption and emission wavelengths. DiR, DiD, DiI and Neuro-DiO can be used in combination for multicolor imaging. DiD is also available in CellBrite™ Red, a ready-to-use cell labeling solution.
<ul>
<li>&lamba;Ex/&lamba;Em (MeOH) = 644/663 nm</li>
<li>&epsilon;= 193,000</li>
<li>Dark blue solid soluble in ethanol, DMF and DMSO</li>
<li>Store at -20°C and protect from light</li>
<li>C₆₇H₁₀₃ClN₂O₃S</li>
<li>MW: 1052.1</li>
<li>[127274-91-3]</li>
</ul>

Descrition & Target

DIG Styramide *Superior Replacement for DIG tyramide*

Descrition & Target

Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (&lambda; _Ex/&lambda; _Em: 355/420nm) in cells, while the oxidized form ethidium has red fluorescence (&lambda; _Ex/&lambda; _Em: 518/605 nm) upon DNA intercalation.<ul><li>Off-white to light brown solid soluble in DMF or DMSO</li><li>Store at -20&deg;C under nitrogen or argon, and protect from light, especially when in solution</li><li>C₂₁H₂₁N₃</li><li>MW: 315</li><li>[38483-26-0]</li></ul>

Descrition & Target

Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Also see dihydrorhodamine 123 dihydrochloride, a more stable and water soluble form of dihydrorhodamine 123.<ul> <li>White solid soluble in DMSO</li> <li>Store at -20&deg;C and protect from air and light, especially when in solution</li> <li>C₂₀H₁₈N₂O₃</li> <li>MW: 346</li> <li>[109244-58-8]</li></ul>

Descrition & Target

Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage.<ul> <li>White solid soluble in DMF</li> <li>Store at -20&deg;C and protect from light, especially in solution</li> <li>C₂₀H₂₀Cl₂N₂O₃</li> <li>MW: 419</li></ul>

Descrition & Target

Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage.<ul> <li>White solid soluble in DMF</li> <li>Store at -20&deg;C and protect from light, especially in solution</li> <li>C₂₀H₂₀Cl₂N₂O₃</li> <li>MW: 419</li></ul>

Descrition & Target

DiIC₁₈(3); 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine

DiI is a widely used carbocyanine membrane dye that labels cell membranes by inserting its two long (C₁₈ carbon) hydrocarbon chains into the lipid bilayer. Particularly, it has been extensively used for the anterograde and retrograde labeling of neurons. The intense fluorescence and high photostability of the dye make it possible to visualize the fine structures (axons and dendrites) of the neurons. Due to its low toxicity and the tendency to give highly stable cell labeling, the dye has also been generally used for long term cell tracing of cells both in cultures and in living embryos or animals. Please also see our ready-to-use CellBrite&trade; Cell Membrane Labeling Solutions.
<ul>
<li>&lamba;_Ex/&lamba; _Em(MeOH) = 549/565 nm</li>
<li>&epsilon; = 148,000</li>
<li>Dark red solid soluble in ethanol, DMF or DMSO</li>
<li>Store at 4°C</li>
<li>C₅₉H₉₇ClN₂O₄</li>
<li>MW: 933.88</li>
<li>[41085-99-8]</li>
</ul>

Descrition & Target

DiO is a carbocyanine membrane dye that has applications similar to those of DiI. However, DiO has green fluorescent emission and the lateral diffusion rate on the membranes is generally slower than that of DiI. DiO and DiI are often used together in dual color studies. Also see Neuro-DiO, which has better solubility in membranes and does not form non-fluorescent aggregates that can slow dye diffusion.
<ul>
<li>&lamba;_Ex/&lamba;_Em (MeOH) = 484/501 nm</li>
<li>&epsilon; (MeOH) = 150,000</li>
<li>Yellow orange solid soluble in ethanol</li>
<li>Store at 4°C and protect from light, especially in solution</li>
<li>C₅₃H₈₅ClN₂O₆</li>
<li>MW: 882</li>
<li>[34215-57-1]</li>
</ul>

Descrition & Target

DiOC₁₄(3) is a DiO derivative, but is more soluble in aqueous media than the latter because it has shorter hydrocarbon chains. Also see our ready-to-use CellBrite&trade; Cell Membrane Labeling Solutions.
<ul>
<li>&lamba;_Ex/&lamba; _Em(MeOH) = 484/501nm</li>
<li>&epsilon;(MeOH) = 150,000</li>
<li>Orange solid soluble in methanol, ethanol or DMSO</li>
<li>Store desiccated at 4°C</li>
<li>C₄₇H₇₄N₂O₆S</li>
<li>MW: 795</li>
</ul>

Descrition & Target

DiOC₆(3) (3,3'-Dihexyloxacarbocyanine,iodide) is a green fluorescent membrane dye that has been used to stain the ER in both live and fixed cells. The dye has also been used to study structural interactions and dynamics of the ER in neurons and yeast. In addition, the dye is widely used as a membrane potential dye.
<ul>
<li>&lamba;_Ex/&lamba;_Em(MeOH) = 484/501 nm</li>
<li>Orange solid soluble in DMSO or DMF</li>
<li>Store at 4°C and protect from light, especially in solution</li>
<li>C₂₉H₃₇IN₂O₂</li>
<li>MW: 572.53</li>
<li>[53213-82-4]</li>
</ul>

Descrition & Target

DiR is a lipophilic, near-infrared fluorescent cyanine dye (Ex/Em 748/780 nm). The dye is useful for labeling cytoplasmic membranes, with two long 18-carbon chains that insert into the cell membrane, resulting in specific and stable cell staining with no or minimal dye transfer between cells.

Descrition & Target

DiR is a lipophilic, near-infrared fluorescent cyanine dye (Ex/Em 748/780 nm). The dye is useful for labeling cytoplasmic membranes, with two long 18-carbon chains that insert into the cell membrane, resulting in specific and stable cell staining with no or minimal dye transfer between cells.

Descrition & Target

Quantitative assay of L-Alanine by colorimetric (570nm) or fluorimetric (530nm/590nm) methods. Procedure: 60 min. Kit size: 100 tests. Detection limit: 0.4 µM. Shelf life: 6 months. Shipping: on ice; storage: -20°C.

Descrition & Target

Quantitative determination of choline by colorimetric (570nm) or fluorometric (530nm/590nm) methods. Procedure: 30 min. Kit size: 100 tests. Detection limit: colorimetric assay 1 µM, fluorimetric assay 0.2 µM. Shelf life: 3 months. Shipping: on ice; storage: -20°C.

Descrition & Target

DMAO is a membrane-permeable DNA dye, excellent for staining live bacteria. DMAO preferentially binds to dsDNA with green fluorescence (Ex/Em (DNA bound) = 503/530 nm). Unlike the SYBR dyes, DMAO is extremely stable and can be routinely handled and stored at room temperature without degradation. Supplied at 2 mM in DMSO.
<ul>
<li>Green DNA stain for live bacteria</li>
<li>Stable for room temperature storage</li>
<li>&lamba;_Ex/&lamba;_Em(with DNA) = 503/530 nm</li>
<li>Supplied at 2 mM in DMSO</li>
<li>Supplied at 1 mL</li>
</ul>