Description & Target

Light-On LysoView™ 555 is a red fluorogenic lysosome dye with pH-dependent fluorescence. Light-On LysoView™ dye is unique among commercially available lysosomotropic dyes in that its fluorescence in cells is UV-activated and reversible.

Description & Target

Light-On LysoView™ 555 is a red fluorogenic lysosome dye with pH-dependent fluorescence. Light-On LysoView™ dye is unique among commercially available lysosomotropic dyes in that its fluorescence in cells is UV-activated and reversible.

Description & Target

The 1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder is composed of chromatography-purified DNA fragments dissolved in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA) at 100 ng/uL. The 1 kb, 3 kb, and 6 kb bands contain more DNA to provide internal orientation. The ladder is supplied with 1.5 mL of 6X  Loading Buffer, which contains density agents and two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel.

Description & Target

1,6-Diphenyl-1,3,5-hexatriene (DPH, excitation/emission=350 nm/420 nm) is almost non-fluorescent in water, but it exhibits a strong increase in fluorescence after intercalation into membranes and can be used as a probe for viscosity, polarity and lipid order. DPH has been used for the high-content imaging of neutral lipid droplets¹ as well as a reporter of inner spore membrane fluidity in bacterial spores².
<ul>
<li>Yellow solid soluble in DMF and DMSO</li>
<li>Store at -20 °C and protect from light</li>
<li>C₁₈H₁₆</li>
<li>MWt: 232.32</li>
<li>[1720-32-7]</li>
</ul>

Description & Target

10-Acetyl-3,7-dihydroxyphenoxazine (N-Acetyl-3,7-dihydroxyphenoxazine or ADHP), which has been marketed under the name Amplex&reg; Red, is regarded as the best fluorogenic substrate for peroxidase because it is highly specific and stable. The substrate itself is nearly colorless and nonfluorescent until it is oxidized by H₂O₂(reacting in a 1:1 stoichiometry) in the presence of horseradish peroxidase (HRP) to become the highly red fluorescent resorufin. H₂O₂ is produced by many enzymatic reactions, and thereforeADHP can be used to detect the activity of many different enzymes.<ul> <li>Detect HRP in immunoassays</li> <li>Detect trace amounts of hydrogen peroxide (H₂O₂)</li> <li>Detect using fluorometer or microplate reader</li> <li>&lambda;_Ex/&lambda;_Em(pH 9)= 571/585 nm (end product)</li> <li>&epsilon;= 54,000 (end product)</li> <li>White to off-white solid soluble in DMSO or DMF</li> <li>Store desiccated at -20&deg;C and protect from light</li> <li>C₁₄H₁₁CNO₄</li> <li>MW: 257.24</li> <li>[119171-73-2]</li></ul>Amplex Red is a registered trademark of Thermo Fisher Scientific.

Description & Target

10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific a4d73786-81ea-96bb-77d4-9ff261b5ff15 binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.

Description & Target

1X Passive Lysis Buffer 2.0 for 9689c5aa-6556-4b3c-b5d1-1e693715ba07’s Firefly & Renilla Luciferase Single Tube Assay Kits (catalog no. 30081) is sold separately for use with the kits when more lysis buffer (e.g. >100 uL/well) is required. It also can be used with recombinant firefly or Renilla luciferase enzymes.

Description & Target

H₂DCFDA (2',7'-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H₂DCFDA becomes the highly green fluorescent 2',7'-dichlorofluorescein.<ul><li>&lambda;_Ex/&lambda; _Em(pH&gt;6) = 504/529 nm (end product)</li><li>White solid soluble in DMSO or DMF</li><li>Store desiccated at -20&deg;C and protect from light</li><li>C₂₄H₁₆Cl₂O₇</li><li>MW: 487.29</li><li>[4091-99-0]</li></ul>

Description & Target

2,3-Diaminonaphthalene reacts with nitrosonium, which is formed from NO, to form the fluorescent dye 1 <i>H</i>-naphthotriazole. This method can be used to detect 10 nM to 10 uM of nitrite (NO₂-) and is compatible with 96-well format. <ul> <li>Yellow orange solid soluble in DMSO</li> <li>Store at desiccated at 4&deg;C, protect from light</li> <li>C₁₀H₁₀N₂</li> <li>MW: 158</li> </ul>

Description & Target

2-((4-Azido-2,3,5,6-tetrafluorobenzoyl)amino)ethyl methanethiosulfonate is a cross-linker containing a thiol-reactive MTS group and a photoaffinity label 4-azido-2,3,5,6-tetrafluorobenzene (ATFB). ATFB is known to be a superior photoaffinity label that undergoes highly efficient C-H bond insertion on UV photolysis. <ul> <li>White solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from light</li> <li>C₁₀H₈F₄N₄O₃S₂</li> <li>MW: 372.32</li> </ul>

Description & Target

2-Sulforhodamine 101 cadaverine can be coupled to activated carboxy groups or sulfonyl chlorides. <ul> <li>&lamba;_Ex/&lamba;_Em(MeOH) = 583/603 nm</li> <li>Dark purple solid soluble in DMSO or DMF</li> <li>Store at 4°C and protect from light</li> <li>C₃₈H₄₃F₃N₄O₈S₂</li> <li>MW: 804.90</li> </ul>

Description & Target

3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate is a water soluble MTS cross-linker. Stock solutions made in anhydrous DMF are stable for at least two months if stored at -20°C. However, stock solutions made in water or buffer should be used immediately.

<ul>

<li>Off-white solid soluble in DMF or water</li>

<li>Store desiccated at -20°C</li>

<li>C₁₂H₂₆O₈S₄</li>

<li>MW: 426.57</li>

</ul>

Description & Target

3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate is a water soluble MTS cross-linker. Stock solutions made in anhydrous DMF are stable for at least two months if stored at -20°C. However, stock solutions made in water or buffer should be used immediately.

<ul>

<li>Off-white solid soluble in DMF or water</li>

<li>Store desiccated at -20°C</li>

<li>C₁₀H₂₂O₇S₄</li>

<li>MW: 382.52</li>

</ul>

Description & Target

3,6-dioxaoctane-1,8-diyl dimethanethiosulfonate is a water soluble MTS cross-linker. Stock solutions made in anhydrous DMF are stable for at least two months if stored at -20°C. However, stock solutions made in water or a buffer should be used immediately.

<ul>

<li>Off-white liquid soluble in DMF or water</li>

<li>Store desiccated at -20°C</li>

<li>C₈H₁₈O₆S₄</li>

<li>MW: 338.49</li>

</ul>

Description & Target

Similar to A-23187, 4-Bromo A-23187 is used for in situ calibrations of fluorescent Ca² indicators to equilibrate intracellular and extracellular calcium concentrations and to allow Mn² to enter cells to quench indicator fluorescence. However, since 4-bromo A-23187 is nonfluorescent, it is preferred over the blue fluorescent A-23187 for calibrations of UV-excited Ca² indicators such as Indo-1 and Fura-2 in order to keep background fluorescence to a minimum.
<ul>
<li>White solid soluble in DMSO or EtOH</li>
<li>Store at 4°C</li>
<li>C₂₉H₃₆BrN₃O₆</li>
<li>MW: 602.52</li>
<li>[76455-48-6]</li>
</ul>