Biotium
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4-Di-1-ASP
Description & Target
4-Di-1-ASP is a styryl dye stains mitochondria of live cells. It has also been used to stain glioma cells in living brain tissues.
<ul>
<li>&lamba;<sub>Ex</sub>/&lamba; <sub>Em</sub> = 474/606 nm (MeOH)</li>
<li>Store at 4°C and protect from light, especially in solution</li>
<li>C<sub>16</sub>H<sub>19</sub>IN<sub>2</sub></li>
<li>MW: 366.2</li>
<li>[68971-03-9]</li>
</ul>
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4-Sulforhodamine 101 cadaverine, trifluoroacete salt
Description & Target
Cyanine NS succinimidyl esters are non-sulfonated cyanine dyes that can be used to label proteins, oligonucleotides and nucleic acids. Available in 3 colors, Cyanine 488NS, Cyanine 555NS, and Cyanine 647NS are non-sulfonated versions of Cy®2, Cy®3, and Cy®5, respectively. They are designed to match by charge and molecular weight for 2D DIGE platform. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 offers these dyes as high quality amine-reactive succinimidyl esters.
<strong>Cyanine 488NS</strong>
<ul>
<li>Ex/Em 483/508 nm</li>
<li>MW: 677</li>
<li>MW added to protein: 437</li>
<li>Orange solid soluble in DMF or DMSO</li>
<li>Store desiccated at -20°C; protect from light</li>
</ul>
<strong>Cyanine 555NS</strong>
<ul>
<li>Ex/Em 547/572 nm</li>
<li>MW: 682</li>
<li>MW added to protein: 441</li>
<li>Red solid soluble in DMF or DMSO</li>
<li>Store desiccated at -20°C; protect from light</li>
</ul>
<strong>Cyanine 647NS</strong>
<ul>
<li>Ex/Em 639/668 nm</li>
<li>MW: 680</li>
<li>MW added to protein: 439</li>
<li>Dark blue solid soluble in DMF or DMSO</li>
<li>Store desiccated at -20°C; protect from light</li>
</ul>
Also see sulfonated Cyanine Dye Succinimidyl Esters and 9689c5aa-6556-4b3c-b5d1-1e693715ba07's CF® Dye Succinimidyl Esters, available with more than 20 bright and photostable CF® Dyes.
Cy dye is a registered trademark of GE Healthcare.
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5(6)-CARBOXYRhodamine 110-C5-MALEIMIDE (
Description & Target
CR110-C5-maleimide (full name: 5(6)-Carboxyrhodamine 110-C5-maleimide) is a maleimide derivative of the green fluorescent dye carboxyrhodamine 110. The dye has spectral properties (&lamba; <sub>Ex</sub>/&lamba; <sub>Em</sub> (MeOH) = 502/524 nm) similar to those of fluorescein (fluorescein-5-maleimide, for example), but the photostability of the dye is among the best of all green fluorescent dyes. Thus, the dye is a superior green fluorescent dye for labeling thiols. <ul> <li>Orange red solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from light, especially in solution</li> <li>C<sub>31</sub>H<sub>30</sub>N<sub>4</sub>O<sub>6</sub></li> <li>MW: 554.59</li> </ul>
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5(6)-CFDA (5-(And-6)-Carboxyfluorescein
Description & Target
5(6)-CFDA (full name: 5-(and-6)-Carboxyfluorescein diacetate) is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein.
<ul>
<li>Off-white solid soluble in DMSO</li>
<li>Store desiccated at 4°C</li>
<li>C<sub>25</sub>H<sub>16</sub>O<sub>9</sub>.</li>
<li>MW: 460</li>
<li>[124387-19-5]</li>
</ul>
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5(6)-CF®DA, SE (5-(AND-6)-CARBOXYFLUORESC
Description & Target
5(6)-CFDA, SE (full name: 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester, also known as CFSE) is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and non-fluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases. The labeling has been shown to be stable to formaldehyde or gluteraldehyde fixation. Also see our CFDA SE Cell Proliferation Assay Kit.
<ul>
<li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em</sub> (of hydrolysis product at pH 7) = 495/519 nm</li>
<li>White solid soluble in DMSO or DMF</li>
<li>Store at -20°C and protect from light</li>
<li>C<sub>29</sub>H<sub>19</sub>NO<sub>11</sub></li>
<li>MW: 557</li>
<li>[150347-59-4]</li>
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5(6)-FAM SE (FULL CHEMICAL NAME: 5-(AND-
Description & Target
5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate. <ul> <li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em</sub> = 495/519 nm</li> <li>Yellow solid soluble in DMSO or DMF</li> <li>Store at -20°C and protect from light</li> <li>C<sub>25</sub>H<sub>15</sub>NO<sub>9</sub></li> <li>MW: 473.4</li> <li>[117548-22-8]</li> </ul>
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5(6)-ROX cadaverine, TFA salt
Description & Target
ROX cadaverine can be coupled to activated carboxy groups or sulfonyl chlorides.
<ul>
<li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em </sub>(MeOH) = 568/595 nm</li>
<li>Dark purple solid soluble in DMSO or DMF</li>
<li>Store at 4°C and protect from light</li>
<li>C<sub>42</sub>H<sub>44</sub>F<sub>6</sub>N<sub>4</sub>O<sub>8</sub></li>
<li>MW: 846.81</li>
</ul>
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5(6)-ROX maleimide
Description & Target
5(6)-ROX maleimide has C6-linker that enhances the fluorescence after the dye is conjugated to proteins. The product is a direct replacement of 5(6)-carboxy-X-rhodamine C5-maleimide (91041). <ul> <li>&lamba; <sub>Ex</sub>/&lamba; <sub>Em</sub> (MeOH) = 568/595 nm</li> <li>Red solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from light, especially in solution</li> <li>C<sub>43</sub>H<sub>44</sub>N<sub>4</sub>O<sub>6</sub></li> <li>MW: 712.81</li> </ul>
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5(6)-Tetramethylrhodamine maleimide
Description & Target
5(6)-Carboxytetramethylrhodamine maleimide has C6-linker that enhances the fluorescence after the dye is conjugated to proteins. The product is a direct replacement for 5(6)-carboxytetramethylrhodamine C5-maleimide (91040) <ul> <li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em</sub> (MeOH) = 540/565 nm</li> <li>Red solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from light, especially in solution</li> <li>C<sub>35</sub>H<sub>36</sub>N<sub>4</sub>O<sub>6</sub></li> <li>MW: 608.66</li> </ul>
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5,5'-Dibromo BAPTA, tetrapotassium salt
Description & Target
BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.
Key advantages of these calcium chelators include relative insensitivity toward intracellular pH change and fast release of calcium. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 offers several BAPTA chelators with calcium dissociation constants covering the biologically significant range from 10<sup>-7</sup> to 10<sup>-2</sup> M.
<ul>
<li>Kd (no Mg<sup>2 </sup>): 3.6 uM</li>
<li>White to off-white solid soluble in water</li>
<li>Store at 4°C</li>
<li>C<sub>22</sub>H<sub>18</sub>Br<sub>2</sub>K<sub>4</sub>N<sub>2</sub>O<sub>10</sub></li>
<li>MW: 786.59</li>
<li>[73630-11-2]</li>
</ul>
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5,5'-Difluoro BAPTA, AM ester
Description & Target
BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.
Key advantages of these calcium chelators include relative insensitivity toward intracellular pH change and fast release of calcium. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 offers several BAPTA chelators with calcium dissociation constants covering the biologically significant range from 10<sup>-7</sup> to 10<sup>-2</sup> M.
Difluoro BAPTA AM ester is a membrane permeable form of Difluoro BAPTA that can be loaded into cells by incubation. Difluoro BAPTA AM ester itself does not bind calcium, but once inside the cell is converted into Difluoro BAPTA by cytoplasmic esterases.
<ul>
<li>Colorless solid soluble in DMSO</li>
<li>Store desiccated at -20°C</li>
<li>C<sub>34</sub>H<sub>38</sub>F<sub>2</sub>N<sub>2</sub>O<sub>18</sub></li>
<li>MW: 801</li>
<li>[156027-00-8]</li>
</ul>
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5,5'-Difluoro BAPTA, tetrapotassium salt
Description & Target
BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.
Key advantages of these calcium chelators include relative insensitivity toward intracellular pH change and fast release of calcium. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 offers several BAPTA chelators with calcium dissociation constants covering the biologically significant range from 10<sup>-7</sup> to 10<sup>-2</sup> M.
<ul>
<li>Kd (no Mg<sup>2 </sup>): 0.59 uM, Kd (1 mM Mg<sup>2 </sup>): 0.70 uM</li>
<li>White solid soluble in water</li>
<li>Store at 4°C</li>
<li>C<sub>22</sub>H<sub>18</sub>F<sub>2</sub>K<sub>4</sub>N<sub>2</sub>O<sub>10</sub></li>
<li>MW: 665</li>
<li>[152290-47-6]</li>
</ul>
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5,5'-Dimethyl BAPTA, AM ester
Description & Target
BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium. Key advantages of these calcium chelators include relative insensitivity toward intracellular pH change and fast release of calcium. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 offers several BAPTA chelators with calcium dissociation constants covering the biologically significant range from 10<sup>-7</sup> to 10<sup>-2</sup> M.
Dimethyl BAPTA AM ester is a membrane permeable form of Dimethyl BAPTA that can be loaded into cells by incubation. Dimethyl BAPTA AM ester itself does not bind calcium, but once inside the cell is converted into Dimethyl BAPTA by cytoplasmic esterases.
<ul>
<li>White to off-white solid soluble in DMSO</li>
<li>Store desiccated at -20°C</li>
<li>C<sub>36</sub>H<sub>44</sub>N<sub>2</sub>O<sub>18</sub></li>
<li>MW: 792.75</li>
<li>[147504-94-7]</li>
</ul>
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5,5'-Dimethyl BAPTA, tetrapotassium salt
Description & Target
BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.
Key advantages of these calcium chelators include relative insensitivity toward intracellular pH change and fast release of calcium. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 offers several BAPTA chelators with calcium dissociation constants covering the biologically significant range from 10<sup>-7</sup> to 10<sup>-2</sup> M.
<ul>
<li>Kd (no Mg<sup>2 </sup>): 0.16 uM, Kd (1 mM Mg<sup>2 </sup>): 0.44 uM</li>
<li>White solid soluble in water</li>
<li>Store at -20°C</li>
<li>C<sub>24</sub>H<sub>24</sub>K<sub>4</sub>N<sub>2</sub>O<sub>10</sub></li>
<li>MW: 657</li>
<li>[73630-09-8]</li>
</ul>
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5-(&6)-Carboxy-2',7'-Dichlorofluorescein
Description & Target
5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)-carboxy-2',7'-dichlorofluorescein by intracellular esterases.
<ul>
<li>White solid soluble in DMSO</li>
<li>Store at 4°C and protect from light, especially in solution</li>
<li>C<sub>25</sub>H<sub>14</sub>Cl<sub>2</sub>O<sub>9</sub></li>
<li>MW: 529</li>
<li>[127770-45-0]</li>
</ul>
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